Saczk, A. A.Okumura, L. L.de Oliveira, M. F.Zanoni, Maria Valnice Boldrin [UNESP]Stradiotto, Nelson Ramos [UNESP]2014-05-202014-05-202006-01-01Chromatographia. Wiesbaden: Vieweg, v. 63, n. 1-2, p. 45-51, 2006.0009-5893http://hdl.handle.net/11449/39661A new methodology was developed for analysis of aldehydes and ketones in fuel ethanol by high-performance liquid chromatography (HPLC) coupled to electrochemical detection. The electrochemical oxidation of 5-hydroxymetkylfurfural, 2-furfuraldehyde, butyraldehyde, acetone and methyl ethyl ketone derivatized with 2,4-dinitrophenylhydrazine (DNPH) at glassy carbon electrode present a well defined wave at +0.94 V; +0.99 V; +1.29 V; +1.15 V and +1.18 V, respectively which are the basis for its determination on electrochemical defector. The carbonyl compounds derivatized were separated by a reverse-phase column under isocratic conditions with a mobile phase containing a binary mixture of methanol /LiClO4(aq) at a concentration of 1.0 x 10(-3) mol L-1 (80:20 v/v) and a flow-rate of 1.1 mL min(-1). The optimum potential for the electrochemical detection of aldehydes-DNPH and ketones-DNPH was +1.0 V vs. Ag/AgCl. The analytical curve of aldehydes-DNPH and ketones-DNPH presented linearity over the range 5.0 to 400.0 ng mL(-1), with detection limits of 1.7 to 2.0 ng mL(-1) and quantification limits from 5.0 to 6.2 ng mL(-1), using injection volume of 20 mu L. The proposed methodology was simple, low time-consuming (15 min/analysis) and presented analytical recovery higher than 95%.45-51engcolumn liquid chromatographyelectrochemical detectionaldehydes and ketones in fuel ethanoldinitrophenylhydrazonesArtigo10.1365/s10337-005-0698-1WOS:000235144500006Acesso restrito00721730180057120000-0002-2296-1393