Humberg, R. M. P.Oshiro, E. T.Cruz, M. do S. P. eRibolla, Paulo Eduardo Martins [UNESP]Alonso, Diego PeresFerreira, A. M. T.Bonamigo, R. A.Tasso, N.Oliveira, A. G. de2016-07-072016-07-072012The American Journal of Tropical Medicine and Hygiene, v. 87, n. 3, p. 470-472, 2012.0002-9637http://hdl.handle.net/11449/141072We investigated the occurrence of Leishmania infantum chagasi in Didelphis albiventris opossums at a wild animal rehabilitation center in the city of Campo Grande, Brazil. A total of 54 opossums were tested for L. i. chagasi infection in peripheral blood and bone marrow samples. The samples were analyzed by direct examination, culturing in a specific medium, and polymerase chain reaction–restriction fragment length polymorphism. Leishmania i. chagasi DNA was detected by polymerase chain reaction–restriction fragment length polymorphism in 11 (20.37%) animals. A total of 81.81% of positive opossums were captured in areas of known visceral leishmaniasis transmission. These results suggest a role for D. albiventris in the urban transmission of visceral leishmaniasis.470-472engBiologia MolecularDiagnostico molecularLeishmaniose VisceralArtigo10.4269/ajtmh.2012.11-0534Acesso restrito35771497484568800000-0001-8735-6090