Dai, LinQi, Yi-PinNiu, Li-NaLiu, YanPucci, Cesar R. [UNESP]Looney, Stephen W.Ling, Jun-QiPashley, David H.Tay, Franklin R.2014-05-202014-05-202011-08-01Crystal Growth & Design. Washington: Amer Chemical Soc, v. 11, n. 8, p. 3504-3511, 2011.1528-7483http://hdl.handle.net/11449/41139Nanocomposites created with polycarboxylic acid alone as a stabilization agent for prenucleation clusters-derived amorphous calcium phosphate exhibit nonperiodic apatite deposition. In the present study, we report the use of inorganic polyphosphate as a biomimetic analog of matrix phosphoprotein for directing poly(acrylic acid)-stabilized amorphous nano-precursor phases to assemble into periodic apatite-collagen nanocomposites. The sorption and desorption characteristics of sodium tripolyphosphate to type I collagen were examined. Periodic nanocomposite assembly with collagen as a template was demonstrated with TEM and SEM using a Portland cement-based resin composite and a phosphate-containing simulated body fluid. Apatite was detected within the collagen at 24 h and became more distinct at 48 h, with prenucleation clusters attaching to the collagen fibril surface during the initial infiltration stage. Apatite-collagen nanocomposites at 72 h were heavily mineralized with periodically arranged intrafibrillar apatite platelets. Defect-containing nanocomposites caused by desorption of TPP from collagen fibrils were observed in regions lacking the inorganic phase.3504-3511engArtigo10.1021/cg200663vWOS:000293356400025Acesso restrito17540206528748500000-0003-4830-0400