Hantao, Leandro WangToledo, Bruna ReginaLima Ribeiro, Fabiana Alves dePizetta, Marilia [UNESP]Pierozzi, Caroline Geraldi [UNESP]Furtado, Edson Luiz [UNESP]Augusto, Fabio2014-12-032014-12-032013-11-15Talanta. Amsterdam: Elsevier Science Bv, v. 116, p. 1079-1084, 2013.0039-9140http://hdl.handle.net/11449/112043In this paper it is reported the use of the chromatographic profiles from volatile fractions of plant clones - in this case, hybrids of Eucalyptus grandis x Eucalyptus urophylla - to determine specimens susceptible to rust disease. The analytes were isolated by headspace solid phase microextraction (HS-SPME) and analyzed by comprehensive two-dimensional gas chromatography combined to fast quadrupole mass spectrometry (GC x GC-qMS). Parallel Factor Analysis (PARAFAC) was employed for estimate the correlation between the chromatographic profiles and resistance against Eucalyptus rust, after preliminary variable selection performed by Fisher ratio analysis. The proposed method allowed the differentiation between susceptible and non-susceptible clones and determination of three resistance biomarkers. This approach can be a valuable alternative for the otherwise time-consuming and laborintensive methods commonly used. (C) 2013 Elsevier B.V. All rights reserved.1079-1084engComprehensive two-dimensional gaschromatographyMass spectrometryMetabolic profileParallel factor analysisPuccinia psidiiResistance biomarkersArtigo10.1016/j.talanta.2013.08.033WOS:000328176000156Acesso restrito38459894858333950000-0002-6924-835X