Di Mauro, Antonio Orlando [UNESP]Di Mauro, Daniela Cristina [UNESP]Capeloto, Andrea [UNESP]Arriel, Nair Helena de CastroCosta, Marcelo Marchi [UNESP]Trevisoli, Sandra Helena UnedaDi Mauro, Sonia Marli Zingaretti [UNESP]2014-05-272014-05-272006-12-01Crop Breeding and Applied Biotechnology, v. 6, n. 2, p. 167-170, 2006.1518-7853http://hdl.handle.net/11449/69276The Random Amplified Polymorphic DNA (RAPD) technique is powerful for DNA polymorphism determinations and is widely used in research involving different organisms, but it is known that RAPD can be affected by many factors that may result in false positive bands and non-reproducible assays. In this study, we analyzed the effect of several factors such as DNA template, primer and Taq DNA polymerase concentrations to optimize and standardize the RAPD technique for further genetic studies with Citrulus lanattus and Sesamum indicum L. The best combination of DNA, Taq DNA polymerase enzyme and primer concentrations in RAPD amplification procedures for sesame and watermelon genotypes was established.167-170engConditions, watermelon, sesameDNA amplificationCitrullus lanatus var. lanatusSesamum indicumArtigo10.12702/1984-7033.v06n02a08Acesso aberto2-s2.0-411491273632-s2.0-41149127363.pdf1275652518822095