Lima, Liliane Spazzapam [UNESP]Weinert, Patricia Los [UNESP]Pezza, Leonardo [UNESP]Pezza, Helena Redigolo [UNESP]2015-03-182015-03-182014-12-10Spectrochimica Acta Part A-molecular And Biomolecular Spectroscopy. Oxford: Pergamon-elsevier Science Ltd, v. 133, p. 597-604, 2014.1386-1425http://hdl.handle.net/11449/116741A flow injection spectrophotometric procedure employing merging zones is proposed for direct bromopride determination in pharmaceutical formulations and biological fluids. The proposed method is based on the reaction between bromopride and p-dimethylaminocinnamaldehyde (p-DAC) in acid medium, in the presence of sodium dodecyl sulfate (SDS), resulting in formation of a violet product (lambda(max) = 565 nm). Experimental design methodologies were used to optimize the experimental conditions. The Beer-Lambert law was obeyed in a bromopride concentration range of 3.63 x 10(-7) to 2.90 x 10(-5) mol L-1, with a correlation coefficient (r) of 0.9999. The limits of detection and quantification were 1.07 x 10(-7) and 3.57 x 10(-7) mol L-1, respectively. The proposed method was successfully applied to the determination of bromopride in pharmaceuticals and human urine, and recoveries of the drug from these media were in the ranges 99.6-101.2% and 98.6-102.1%, respectively. This new flow injection procedure does not require any sample pretreatment steps. (C) 2014 Elsevier B.V. All rights reserved.597-604engBromoprideFlow injection analysisPharmaceuticalsUrineArtigo10.1016/j.saa.2014.06.027WOS:000340330900077Acesso restrito597890859185352431921250983611210000-0003-0197-7369