Vieira, José Cavalcante Souza [UNESP]de Oliveira, Grasieli [UNESP]Braga, Camila Pereirada Silva Fernandes, Milenide Moraes, Paula MartinBuzalaf, Marília Afonso Rabelode Oliveira, Lincoln Carlos Silvade Magalhães Padilha, Pedro [UNESP]2020-12-122020-12-122020-10-01Biological Trace Element Research, v. 197, n. 2, p. 667-675, 2020.1559-07200163-4984http://hdl.handle.net/11449/198389Recent studies have demonstrated the association of mercury (Hg) with some fish proteins, milk, and hair from individuals exposed to the element in the Amazon. However, few studies involve identifying biomarkers of mercury exposure. Therefore, the present study aimed to identify potential biomarkers of Hg exposure in fish. For this, the muscular tissues of two species of fish (Prochilodus lineatus and Mylossoma duriventre) that feed the Amazonian human population were analyzed. Through the analyses obtained by graphite furnace atomic absorption spectrometry (GFAAS), it was possible to identify four protein SPOTS where mercury was present. These SPOTS, identified by mass spectrometry (ESI-MS/MS), included parvalbumin and ubiquitin-40S ribosomal protein S27a, and these being metalloproteins with biomarker characteristics. In addition, the results show the intense Hg/protein ratio observed in the two proteins, which makes metalloproteins strong candidates for biomarkers of mercury exposure. [Figure not available: see fulltext.].667-675engBiomarkersFishMercury in the AmazonMercury-bound proteinsMetalloproteinsProteomicsArtigo10.1007/s12011-020-02026-w2-s2.0-85077711774