Leal, Luciana da Silva [UNESP]Moya-Araújo, Carla Fredrichsen [UNESP]Fernandes, Cláudia Barbosa [UNESP]Martins, Lilian Rigatto [UNESP]Landim-Alvarenga, Fernanda da Cruz [UNESP]Oba, Eunice [UNESP]2016-07-072016-07-072010Revista Veterinaria, v. 21, suplemento 1, p. 892-894, 2010.1668-4834http://hdl.handle.net/11449/141311Ovaries of 86 buffaloes and 95 cows were collected from slaughterhouses and transported to the laboratory in saline solution at 36o C. The Cumulus-oocyte complexes (COCs) were recovered by follicular aspiration and only grades I and II COCs were selected and matured in TCM 199 supplemented with 10% fetal calf serum, sodium pyruvate, LH, FSH, estradiol, gentamicin and cysteamin, for 22-24 hours. A total of 714 and 1983 COCs were recovered from buffaloes and cows, respectively. In the buffaloes, the recovery rates of each COCs categories (grade I: 25.9%; grade II: 30.7%; grade III: 10.2%; denuded 18.6% and expanded: 14.6%) were lower than cows (31.8; 30.6; 15.4; 4.5; 17.7%, respectively) according to Mann-Whitney Test (p< 0.05). The percentage of bubaline oocytes that reached metaphase II (63.4% - 242/ 396) was lower than bovine oocytes (67.8% - 696/ 1234) under the same laboratory conditions. These differences observed in all analysis realized indicate that each species has peculiar phisiological characteristics.892-894engBuffaloesCowsIn vitro maturationOocytesArtigoAcesso restrito9224380586557664