Santos, I. WalterBinsfeld, L. C.Weiss, R. R.Kozicki, L. E.Rochadelli, R.Resende, M. V. [UNESP]Hossepian de Lima, V. F M [UNESP]2014-05-272014-05-272007-12-31Archives of Veterinary Science, v. 12, n. 2, p. 47-54, 2007.1517-784Xhttp://hdl.handle.net/11449/70229The objective of the present study was to compare the in vitro and in vivo profile of frozen dog semen with Tris-bovine serum albumin (TB) and Tris-egg yolk (TE) extenders. Twenty dogs were used as donors. Each dog was stimulated by penile massage and only the sperm-rich fraction was collected weekly until 40 ejaculates were obtained. After macroscopic and microscopic analysis, equal parts of each ejaculate were diluted with TB and TE by the one-step method at 37 °C. The semen was added to 0.5-mL French straws which presented normal characteristics before freezing and after thawing. Acrosomal integrity was evaluated by double Trypan blue-Giemsa staining, in which alive intact (LI), alive reacted (LR), dead intact (DI) and dead reacted (DR) spermatozoa, were identified by the time of thawing and up to 4 h of incubation at 39 °C, the TE being significantly superior to TB (P<0,01) in the LI and LR variables. The TB being significantly superior to TE (P<0,01) in the DR variable. Female dogs in natural heat were submitted to artificial insemination, 20 receiving TE-semen and 20 receiving TB-semen with the Osiris probe (IMV, L'Aigle, France) and the numbers indicate that TE was significantly better than TB (P<0,01) to pregnancy rate and number of puppies/delivery. We concluded from this study, that TE was better than TB, because this, induced an eady acrossome reaction in dog's sperm.47-54engBitchExtendersOestrusSemenBovinaeCanis familiarisOsirisArtigoAcesso aberto2-s2.0-373490172602-s2.0-37349017260.pdf