Marinotti, OsvaldoCerqueira, Gustavo C.Almeida, Luiz Gonzaga Paula deFerro, Maria Inês Tiraboschi [UNESP]Loreto, Elgion Lucio da SilvaZaha, ArnaldoTeixeira, Santuza M. R.Wespiser, Adam R.Silva, Alexandre Almeida E.Schlindwein, Aline DaianePacheco, Ana Carolina LandimSilva, Artur Luiz da Costa daGraveley, Brenton R.Walenz, Brian P.Lima, Bruna de AraújoRibeiro, Carlos Alexandre GomesNunes-Silva, Carlos GustavoCarvalho, Carlos Roberto deSoares, Célia Maria de AlmeidaMenezes, Claudia Beatriz Afonso deMatiolli, CleversonCaffrey, DanielAraújo, Demetrius Antonio M.Oliveira, Diana Magalhães deGolenbock, DouglasGrisard, Edmundo CarlosFantinatti-Garboggini, FabianaCarvalho, Fabíola Marques deBarcellos, Fernando GomesProsdocimi, FranciscoMay, GemmaAzevedo Junior, Gilson Martins deGuimarães, Giselle MouraGoldman, Gustavo HenriquePadilha, Itácio Q. M.Batista, Jacqueline da SilvaFerro, Jesus Aparecido [UNESP]Ribeiro, José M. C.Fietto, Juliana Lopes RangelDabbas, Karina Maia [UNESP]Cerdeira, LouiseAgnez-Lima, Lucymara FassarellaBrocchi, MarceloCarvalho, Marcos Oliveira deMelo Teixeira, Marcus deMascena Diniz Maia, Maria deGoldman, Maria Helena S.Schneider, Maria Paula CruzFelipe, Maria Sueli SoaresHungria, MariangelaNicolás, Marisa FabianaPereira, MaristelaMontes, Martín AlejandroCantão, Maurício E.Vincentz, MichelRafael, Miriam SilvaSilverman, NealStoco, Patrícia HermesSouza, Rangel CelsoVicentini, RenatoGazzinelli, Ricardo TostesOliveira Neves, Rogério deSilva, RosaneAstolfi-Filho, SpartacoMaciel, Talles Eduardo FerreiraÜrményi, Turàn P.Tadei, Wanderli PedroCamargo, Erney PlessmannVasconcelos, Ana Tereza Ribeiro de2014-05-272014-05-272013-08-01Nucleic Acids Research, v. 41, n. 15, p. 7387-7400, 2013.0305-10481362-4962http://hdl.handle.net/11449/76152Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vectorhuman and vectorparasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles- darlingi. © 2013 The Author(s).7387-7400engAnophelesAnopheles darlingiAnopheles gambiaecircadian rhythmfemalegene identificationgene sequencegenetic conservationgenetic markergenome analysisgenome sizeindel mutationinsect geneticsinsecticide resistancemalenonhumanpriority journalsequence homologysingle nucleotide polymorphismsyntenytransposonxenobiotic metabolismArtigo10.1093/nar/gkt484WOS:000323970700025Acesso restrito2-s2.0-8488347143201472417236124647587208482384059