Diazepam, em dose única, inibe a migração celular, a estimulação macrofágica e a atividade de TNF-α na reação inflamatória aguda induzida por LPS em camundongos

Monteiro, Diana AmaralCarlos, Iracilda Zeppone [UNESP]Pinto, Fábio Gonçalves2014-05-272014-05-272008-10-01Revista Brasileira de Ciências Farmaceuticas. São Paulo: Univ São Paulo, Conjunto Quimicas, v. 44, n. 4, p. 613-620, 2008.1516-9332http://hdl.handle.net/11449/130436Benzodiazepines are one of the most frequently prescribed drugs due to their anxiolytic properties. The aim of this study was to evaluate the effects of diazepam on lipopolysaccharide-induced peritoneal acute inflammatory responses. Swiss mice were treated with diazepam in a single dose of 1 or 10 mg/kg- subcutaneously 1 h before an intraperitoneal injection of lipopolysaccharide or sterile saline solution. The mice were killed 16 h after and the cells were washed from the peritoneal cavity to determine the total number of cells and the mononuclear and polimorfonuclear subpopulations, as well as the TNF-alpha activity and percentage of spread macrophages. Our results showed that the diazepam treatment (1 and 10 mg/kg) induced a significant reduction in the LPS-induced macrophage stimulation and TNF-α activity. Diazepam (10 mg/kg) also reduced the inflammatory cellular migration when compared to the control. It can be concluded that the diazepam treatment in a single dose is able to influence the inflammatory cellular influx, macrophage stimulation and TNF-α activity in the acute inflammatory response in mice, having possible implications on the anti-infectious response efficiency.613-620porAcute inflammatory responseCellular migration/inhibitionDiazepamLipopolysaccharideMacrophages/stimulationTnf-αDiazepamTumor necrosis factor alphaAnimal cellAnimal experimentAntiinflammatory activityAscites fluid cytologyCell countChemotaxisControlled studyDrug dose comparisonDrug effectDrug mechanismFemaleInflammationInflammatory cellMacrophageMacrophage migrationMononuclear cellMouseNonhumanPolymorphonuclear cellSingle drug doseSwiss webster mouseDiazepam, em dose única, inibe a migração celular, a estimulação macrofágica e a atividade de TNF-α na reação inflamatória aguda induzida por LPS em camundongosDiazepam, in a single dose, inhibits cellular chemotaxis, macrophage stimulation, and TNF-α activity in LPS-induced acute inflammatory responses in miceArtigo10.1590/S1516-93322008000400007S1516-93322008000400007WOS:000263804500007Acesso aberto2-s2.0-596491195352-s2.0-59649119535.pdf1730146818754269