Vieira, Gabrielle [UNESP]Khalil, Zeinab G.Capon, Robert J.Sette, Lara D. [UNESP]Ferreira, Henrique [UNESP]Sass, Daiane C. [UNESP]2022-05-012022-05-012022-04-01Journal of Applied Microbiology, v. 132, n. 4, p. 3081-3088, 2022.1365-26721364-5072http://hdl.handle.net/11449/233970Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment. Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml–1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis. Conclusion: Penicillic acid can be an alternative against citrus canker. Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.3081-3088engagricultureantimicrobialsbioproductsbiotechnologyplant diseasesArtigo10.1111/jam.154132-s2.0-85122295334