Foresti, F. [UNESP]Oliveira, C. [UNESP]Foresti de Almeida-Toledo, L. [UNESP]2014-05-272014-05-271993-09-15Experientia, v. 49, n. 9, p. 810-813, 1993.0014-4754http://hdl.handle.net/11449/130469This paper describes a new technique for preparing mitotic fish chromosomes using short-term in vitro treatment with colchicine. The results show that a large number of good quality metaphases (many suitable for chromosome banding) can be obtained by this technique, which requires an average of 1 h and 30 min for all steps. The procedure considerably reduces the time normally required for chromosome preparations in fish.810-813engfish cytogeneticsmitotic chromosomestechniquecolchicineanimal tissuechromosomecytogeneticsfishmitosisnonhumanpriority journalArtigo10.1007/BF01923555WOS:A1993LY25500015Acesso restrito2-s2.0-00273666580804793944846367