Buratini, J.Glapinski, V. F.Giometti, I. C.Teixeira, A. B.Costa, I. B.Avellar, MCWBarros, C. M.Price, C. A.2014-05-202014-05-202005-03-01Molecular Reproduction and Development. Hoboken: Wiley-liss, v. 70, n. 3, p. 255-261, 2005.1040-452Xhttp://hdl.handle.net/11449/17440Paracrine cell signaling is thought to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family have been suggested. In the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR-3c and FGFR-4) are expressed in bovine preantral follicles. Reverse transcription-polymerase chain reaction was used to amplify bovine FGF-8, FGFR-3c, and FGFR-4 from preantral follicle samples and a variety of fetal and adult tissues. All three genes were widely expressed in fetal tissues, with a restricted expression pattern in adult tissues. FGF-8 and FGFR-3c were expressed in secondary follicles in 70% of fetuses examined, whereas FGFR-4 expression was significantly less frequent (20%). FGFR-3c expression frequency was significantly lower in primordial compared to secondary follicles, and FGF-8 expression showed a similar trend. FGFR-4 was only observed when all follicle classes of an individual were expressing both FGF-8 and FGFR-3c. We conclude that FGF-8 and its receptors are expressed in preantral follicles in a developmentally regulated manner. (C) 2005 Wiley-Liss, Inc.255-261engfibroblast growth factor-8fibroblast growth factor receptor-3cfibroblast growth factor receptor-4preantral follicleovaryArtigo10.1002/mrd.20205WOS:000226695700002Acesso restrito7795883009987806