Damazo, Amilcar SabinoMoradi-Bidhendi, NiloufarOliani, Sonia Maria [UNESP]Flower, Roderick John2014-05-272014-05-272007-07-01Birth Defects Research Part A - Clinical and Molecular Teratology, v. 79, n. 7, p. 524-532, 2007.1542-07521542-0760http://hdl.handle.net/11449/69728BACKGROUND: Annexin 1 is a 37-kDa protein that has complex intra- and extracellular effects. To discover whether the absence of this protein alters bone development, we monitored this event in the annexin-A1 null mice in comparison with littermate wild-type controls. METHODS: Radiographic and densitometry methods were used for the assessment of bone in annexin-A1 null mice at a gross level. We used whole-skeleton staining, histological analysis, and Western blotting techniques to monitor changes at the tissue and cellular levels. RESULTS: There were no gross differences in the appendicular skeleton between the genotypes, but an anomalous development of the skull was observed in the annexin-A1 null mice. This was characterized in the newborn annexin-A1 null animals by a delayed intramembranous ossification of the skull, incomplete fusion of the interfrontal suture and palatine bone, and the presence of an abnormal suture structure. The annexin-A1 gene was shown to be active in osteocytes during this phase and COX-2 was abundantly expressed in cartilage and bone taken from annexin-A1 null mice. CONCLUSIONS: Expression of the annexin-A1 gene is important for the normal development of the skull in mice, possibly through the regulation of osteoblast differentiation and a secondary effect on the expression of components of the cPLA2-COX-2 system. © 2007 Wiley-Liss, Inc.524-532engAnnexin 1BoneCyclooxygenase-2Cytosolic phospholipase A2Osteoblastannexinannexin 1cyclooxygenase 2phospholipase A2proteinunclassified drugadolescentanimal tissueannexin a1 genebone densitycontrolled studycraniofacial developmentfemalegenegene activationhistologyimmunohistochemistrymalemousenewbornnonhumanossificationosteocytepriority journalprotein expressionskull sutureWestern blottingAnimalsAnimals, NewbornAnnexin A1Bone and BonesBone DensityBone DevelopmentCraniofacial AbnormalitiesCyclooxygenase 2FemaleGene ExpressionHomozygoteMaleMiceMice, Inbred C57BLMice, KnockoutOsteogenesisPhospholipases AAnimaliaMusArtigo10.1002/bdra.20368Acesso restrito2-s2.0-344475040235102737730539655