Vasconcellos, Luana M. R. [UNESP]Villaça-Carvalho, Maria F. L. [UNESP]Prado, Renata F. [UNESP]Santos, Evelyn L. S. L. [UNESP]Regone, Natal N. [UNESP]Pereira, Marinalda C. [UNESP]Codaro, Eduardo N. [UNESP]Acciari, Heloisa A. [UNESP]2018-11-262018-12-112018-11-262018-12-112017-05-01Journal of Engineering Science and Technology, v. 12, n. 5, p. 1240-1252, 2017.1823-4690http://hdl.handle.net/11449/180594Titanium and some of its alloys exhibit excellent anti-corrosive and biocompatibility properties due to rapid formation of a passive film on their surfaces when exposed to the atmosphere. However, such materials present poor osteoindutive properties. Surfaces modified via anodization are being proposed in this study to promote a chemical interaction between implants and bone cells. For this purpose, samples in Ti-6Al-4V alloy discs were anodized in a phosphoric acid solution using pulsed current for being applied in orthopaedic implants. The pulsed current is based on duty cycle (DC), which was supplied by a square wave pulse rectifier at 100 Hz and maximum tension of 30 V. A scanning electron microscope was used to obtain images of the anodized surfaces, thus revealing the presence of uniformly distributed pores over the entire surface, measuring approximately 2 mu m in diameter. Osteogenic cells grown on the surface of the control and anodized samples were assayed for cytotoxicity and mineralized matrix formation. The anodized surfaces presented a higher rate of viable cells after 10 days, as well as a higher amount of nodules (p = 0.05). In conclusion, these results suggest that the nanotopography promoted by anodization using pulsed current induces beneficial modulatory effects on osteoblastic cells.1240-1252engAnodizingOsseointegrationOsteoblast activityTi-6Al-4V alloyArtigoWOS:000419679700008Acesso restrito2-s2.0-85019712313