Nuernberg, Marta Aparecida Alberton [UNESP]Wainwright, MarkMiessi, Daniela Maria Janjacomo [UNESP]Scalet, Vitor [UNESP]Olivo, Mariane Bocalon [UNESP]Ervolino, Edilson [UNESP]Garcia, Valdir Gouveia [UNESP]Theodoro, Letícia Helena [UNESP]2020-12-122020-12-122020-09-01Photodiagnosis and Photodynamic Therapy, v. 31.1873-15971572-1000http://hdl.handle.net/11449/201916Aim: This study evaluated three concentrations of butyl toluidine blue (BuTB) for antimicrobial photodynamic therapy (aPDT) in experimental periodontitis (EP) in rats. Material and methods: EP was ligature-induced at the first mandibular molar in 105 rats. Ligature was removed after 7 days and animals were distributed into the following treatments: SRP, scaling and root planing (SRP) plus saline solution; BuTB-0.1, SRP plus BuTB at 0.1 mg/mL; aPDT-0.1, SRP plus BuTB at 0.1 mg/mL and InGaAlP diode laser (DL) irradiation; BuTB-0.5, SRP plus BuTB at 0.5 mg/mL; aPDT-0.5, SRP plus BuTB at 0.5 mg/mL and DL irradiation; BuTB-2.0, SRP plus BuTB at 2 mg/mL; aPDT-2.0, SRP plus BuTB at 2 mg/mL and DL irradiation. Five animals from each group were submitted to euthanasia at 7, 15 and 30 days post-treatment. The furcation area was submitted to histological, histometric and immunohistochemical (TGF-ß1, OCN and TRAP) analyses. Results: aPDT-0.5 group presented a better tissue remodeling in all periods, resolution of the inflammatory response and bone neoformation areas at 30 days. aPDT-0.5 also resulted in higher immunolabeling patterns of TGF-ß1 at all periods (p < 0.05) and of OCN at 30 days (p < 0.05). Conclusion: aPDT-0.5 showed the best benefits for inflammatory response and periodontal repair process.engAnimal disease modelDental scalingPeriodontitisPhotochemotherapyPhotosensitizersArtigo10.1016/j.pdpdt.2020.1018682-s2.0-85087077607