de Oliveira, Sarah Gomes [UNESP]de Moura, Rita CassiaMartins, Cesar [UNESP]2014-05-202014-05-202012-11-06Bmc Genetics. London: Biomed Central Ltd., v. 13, p. 6, 2012.1471-2156http://hdl.handle.net/11449/18746Background: To contribute to the knowledge of coleopteran cytogenetics, especially with respect to the genomic content of B chromosomes, we analyzed the composition and organization of repetitive DNA sequences in the Coprophanaeus cyanescens karyotype. We used conventional staining and the application of fluorescence in situ hybridization (FISH) mapping using as probes C(0)t-1 DNA fraction, the 18S and 5S rRNA genes, and the LOA-like non-LTR transposable element (TE).Results: The conventional analysis detected 3 individuals (among 50 analyzed) carrying one small metacentric and mitotically unstable B chromosome. The FISH analysis revealed a pericentromeric block of C(0)t-1 DNA in the B chromosome but no 18S or 5S rDNA clusters in this extra element. Using the LOA-like TE probe, the FISH analysis revealed large pericentromeric blocks in eight autosomal bivalents and in the B chromosome, and a pericentromeric block extending to the short arm in one autosomal pair. No positive hybridization signal was observed for the LOA-like element in the sex chromosomes.Conclusions: The results indicate that the origin of the B chromosome is associated with the autosomal elements, as demonstrated by the hybridization with C(0)t-1 DNA and the LOA-like TE. The present study is the first report on the cytogenetic mapping of a TE in coleopteran chromosomes. These TEs could have been involved in the origin and evolution of the B chromosome in C. cyanescens.6engChromosomal rearrangementsHeterochromatinMultigene familiesSupernumerary chromosomeTransposable elementsArtigo10.1186/1471-2156-13-96WOS:000312340700001Acesso abertoWOS000312340700001.pdf88588006994253520000-0003-3534-974X