Acosta, Daiane da SilvaKneip, Flavia CondeDe Almeida, Eduardo Alves [UNESP]Ventura-Lima, JulianeMonserrat, Jose MariaGeracitano, Laura Alicia2014-05-202014-05-202012-10-01Fish Physiology and Biochemistry. Dordrecht: Springer, v. 38, n. 5, p. 1477-1485, 2012.0920-1742http://hdl.handle.net/11449/22257The carbon nanomaterial fullerene (C-60) can act as anti or pro-oxidant. The aim of this study was to evaluate, in cell suspensions of carp brains (Cyprinus carpio, Cyprinidae), the effect of C-60 after a pre-treatment with polyunsaturated fatty acid (PUFAs) such as omega-3 (docosahexaenoic acid, DHA) and omega-6 (linoleic acid, LA). Assays consisted of a pre-treatment with PUFA (48 h) and then exposure to C-60 (2 h). Cell viability and total anti-oxidant capacity did not differ (p > 0.05). A reduction (p < 0.05) was observed in reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) concentration in fish brain cells pre-exposed with PUFA groups and then exposed or not with C-60. An antioxidant effect of C-60 was evident since in control group (cells not pre-exposed to PUFA), a significant (p < 0.05) reduction of intracellular ROS concentration was observed, although this reduction was not enough to reduce the TBARS levels. Cysteine levels presented a reduction (p < 0.05) in all groups exposed to C-60. For glutathione (GSH), an increase (p < 0.05) was registered in cells exposed to C-60 without PUFAs pre-treatment and in the C-60 group pre-treated with DHA. Overall C-60 appears to play an antioxidant role that is modulated by PUFA, taking into account its effects on intracellular ROS concentration and MDA levels. Results also suggest that C-60 influences GSH synthesis, as showed for the augmented levels of this antioxidant and also for the lowering of the intracellular cysteine concentration.1477-1485engFullerenePolyunsaturated acidsNanoparticlesFish brainCysteineGlutathioneArtigo10.1007/s10695-012-9635-zWOS:000308968000022Acesso restrito