Harvey, P. C.Watson, M.Hulme, S.Jones, M. A.Lovell, M.Berchieri, Jr. [UNESP]Young, J.Bumstead, N.Barrow, P.2014-05-272014-05-272011-10-01Infection and Immunity, v. 79, n. 10, p. 4105-4121, 2011.0019-95671098-5522http://hdl.handle.net/11449/72708The pattern of global gene expression in Salmonella enterica serovar Typhimurium bacteria harvested from the chicken intestinal lumen (cecum) was compared with that of a late-log-phase LB broth culture using a whole-genome microarray. Levels of transcription, translation, and cell division in vivo were lower than those in vitro. S. Typhimurium appeared to be using carbon sources, such as propionate, 1,2-propanediol, and ethanolamine, in addition to melibiose and ascorbate, the latter possibly transformed to D-xylulose. Amino acid starvation appeared to be a factor during colonization. Bacteria in the lumen were non- or weakly motile and nonchemotactic but showed upregulation of a number of fimbrial and Salmonella pathogenicity island 3 (SPI-3) and 5 genes, suggesting a close physical association with the host during colonization. S. Typhimurium bacteria harvested from the cecal mucosa showed an expression profile similar to that of bacteria from the intestinal lumen, except that levels of transcription, translation, and cell division were higher and glucose may also have been used as a carbon source. © 2011, American Society for Microbiology.4105-4121engarginineascorbic acidcarboncitrate synthaseDNA binding proteinDNA directed DNA polymerase gammaethanolamineflagellinglucosehost factor 1melibiosepropionic acidpropylene glycolputrescine derivativethreonine dehydratasexyluloseamino acid metabolismamino acid transportanimal tissuebacterial colonizationcarbon sourcececumcell divisionchromosome replicationcontrolled studycross reactionDNA replicationdown regulationenergy yieldflagellumgene controlgene expressiongenetic transcriptionin vitro studyin vivo studyintestineintestine mucosanonhumanpathogenicitypriority journalSalmonella entericaSalmonella typhimuriumupregulationvirulenceAnimalsBacterial ProteinsCecumChickensGene Expression ProfilingGene Expression Regulation, BacterialGenome, BacterialMiceMice, Inbred BALB COligonucleotide Array Sequence AnalysisPoultry DiseasesSalmonella Infections, AnimalSpecific Pathogen-Free OrganismsUp-RegulationVirulenceVirulence FactorsArtigo10.1128/IAI.01390-10Acesso restrito2-s2.0-808551365722-s2.0-80855136572.pdf