Vicari, M. R.Pansonato-Alves, J. C. [UNESP]Foresti, F. [UNESP]2023-07-292023-07-292015-01-01Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 144-165.http://hdl.handle.net/11449/247569The principle of chromosome microdissection technique is based on the identification of either a chromosome, chromosome arm or chromosome band for the subsequent removal of such target with the aid of a micromanipulator attached to an inverted microscope. Once the target is defined, whole chromosomes, or chromosomal regions, are directly isolated under an inverted microscope linked to a micromanipulator. Following dissection, chromosomal fragments pass through a step of amplification, using adapted methods of whole genome amplification (WGA) in order to increase the limited amount of DNA (Telenius et al. 1992; Zhang et al. 1992) and reduce the starting copies material (Fig. 1).144-165engCapítulo de livro10.1201/b18534-212-s2.0-85075122135