Singulani, Junya L [UNESP]Scorzoni, Liliana [UNESP]Da Silva, Patricia B [UNESP]Nazaré, Ana C [UNESP]Polaquini, Carlos R [UNESP]Baveloni, Franciele G [UNESP]Chorilli, Marlus [UNESP]Regasini, Luis O [UNESP]Fusco-Almeida, Ana M [UNESP]Mendes-Giannini, Maria J.S. [UNESP]2022-05-012022-05-012022-03-01Future Microbiology, v. 17, n. 4, p. 281-291, 2022.1746-09211746-0913http://hdl.handle.net/11449/234170Aim: Octyl gallate (OG) loaded into a nanostructured lipid system (NLS) was tested for antifungal activity and in vitro and in vivo toxicity. Methods & Results: The features of NLS-OG were analyzed by dynamic light scattering and showed adequate size (132.1 nm) and homogeneity (polydispersity index = 0.200). OG was active against Paraccoccidioides spp., and NLS-OG did not affect antifungal activity. NLS-OG demonstrated reduced toxicity to lung cells and zebrafish embryos compared with OG, whereas NLS was toxic to hepatic cells. OG and NLS-OG did not show toxicity in a Galleria mellonella model at 20 mg/kg. All toxic concentrations were superior to MIC (antifungal activity). Conclusion: These results indicate good anti-Paracoccidioides activity and low toxicity of NLS-OG.281-291engantifungal activitynanoparticlesnonconventional animal modelssystemic mycosistoxicityArtigo10.2217/fmb-2021-00952-s2.0-85125020725