Oliveira, Ruberney S.Biscaia, Stellee M. P.Bellan, Daniel L.Viana, Sthefany R. F. [UNESP]Di-Medeiros Leal, Maria CarolinaVasconcelos, Ana Flora D. [UNESP]Liao, Luciano M.Trindade, Edvaldo S.Carbonero, Elaine R.2019-10-042019-10-042019-12-01Carbohydrate Polymers. Oxford: Elsevier Sci Ltd, v. 225, 9 p., 2019.0144-8617http://hdl.handle.net/11449/186171A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects. FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17. The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments. The results indicated that FMG-Hm contained a alpha-(1 -> 6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of alpha-L-fucopyranose and beta-D-mannopyranose. The predicted structure of the heteropolysaccharide was established as: [GRAPHICS] .9engHypsizygus marmoreusMushroomFucomannogalactanChemical structureAntimelanomaB16-F10 cellArtigo10.1016/j.carbpol.2019.115203WOS:000485109100052Acesso aberto