Barros, Aruana RochaAdorno, Maria Ângela TalaricoSakamoto, IsabelMaintinguer, Sandra Imaculada [UNESP]Varesche, Maria Bernadete AmâncioSilva, Edson Luiz2015-05-152015-05-152011Bioresource Technology, v. 102, n. 4, p. 3840-3847, 2011.0960-8524http://hdl.handle.net/11449/123575This study evaluated two different support materials (ground tire and polyethylene terephthalate [PET]) for biohydrogen production in an anaerobic fluidized bed reactor (AFBR) treating synthetic wastewater containing glucose (4000 mg L1 ). The AFBR, which contained either ground tire (R1) or PET (R2) as support materials, were inoculated with thermally pretreated anaerobic sludge and operated at a temperature of 30 C. The AFBR were operated with a range of hydraulic retention times (HRT) between 1 and 8 h. The reactor R1 operating with a HRT of 2 h showed better performance than reactor R2, reaching a maximum hydrogen yield of 2.25 mol H2 mol1 glucose with 1.3 mg of biomass (as the total volatile solids) attached to each gram of ground tire. Subsequent 16S rRNA gene sequencing and phylogenetic analysis of particle samples revealed that reactor R1 favored the presence of hydrogen-producing bacteria such as Clostridium, Bacillus, and Enterobacter.3840-3847engHydrogen Bioproductionanaerobic fluidized bed reactorground tirePET16s rRNAArtigo10.1016/j.biortech.2010.12.014Acesso abertoISSN0960-8524-2011-102-04-3840-3847.pdf2967035823175406972027421457337122910547691946650000-0002-4584-7649