Rinaldo, Daniel [UNESP]Batista, Joao M. [UNESP]Rodrigues, Juliana [UNESP]Benfatti, Ana C. [UNESP]Rodrigues, Clenilson M. [UNESP]Santos, Lourdes Campaner dos [UNESP]Furlan, Maysa [UNESP]Vilegas, Wagner [UNESP]2014-05-202014-05-202010-08-01Chirality. Hoboken: Wiley-liss, v. 22, n. 8, p. 726-733, 2010.0899-0042http://hdl.handle.net/11449/26246When catechins are found in plant extracts, they are almost always identified as catechin and/or epicatechin probably due to stereoselectivity of the enzymes involved in the biosynthesis of these substances. However, the lack of reports regarding to ent-catechin as well as ent-epicatechin does not necessarily mean that these compounds have not been produced. In fact, most of the previous reports used chromatographic conditions not suitable for such separation. This article describes a simple and reliable analytical HPLC-PAD-CD method for simultaneous determination of catechin diastereomers both in infusions and extracts from the leaves of Byrsonima species. The direct separation of catechin, ent-catechin, epicatechin, and ent-epicatechin was obtained in normal phase by HPLC-PAD-CD using Chiralcel OD-H as chiral stationary phase and n-hexane/ethanol with 0.1% of TFA as mobile phase. Chirality 22: 726-733, 2010. (C) 2010 Wiley-Liss, Inc.726-733engchiral chromatographyenantioseparationcircular dichroismplant extractArtigo10.1002/chir.20824WOS:000280056100003Acesso restrito7927877224326837353825364060297713080427947868720000-0003-3032-2556