Vidal, Isabelle GoncalvesTescarollo Dias, Iara Luciade Oliveira Neto, Gracilianode Vasconcellos Lanza, Marcos RobertoTaboada Sotomayor, Maria Del Pilar [UNESP]2014-05-202014-05-202009-01-01Analytical Letters. Philadelphia: Taylor & Francis Inc, v. 42, n. 2, p. 352-365, 2009.0003-2719http://hdl.handle.net/11449/41385A rapid and effective method for carbamide peroxide (CP) quantification in pharmaceuticals is proposed. The reagentless biosensor was prepared by using lyophilized turnip extract as horseradish peroxidase (HRP) enzyme source. The biosensor presented the best performance. The measurements were carried out in 0.1mol L-1 Pipes buffer (pH 6.0). At-100mV vs. Ag|AgCl, a linear response range from 3.0 to 25.0mmol L-1 was obtained. The quantification and detection limits were 1.2 and 0.4mmol L-1, respectively, and the response time was 0.5s. The biosensor repeatability, storage, and lifetime were excellent, allowing a satisfactory CP quantification in real pharmaceutical samples, when compared with those obtained by the official method.352-365engAmperometric biosensorbrassica napus Lcarbamide peroxideHRPlyophilization procedureArtigo10.1080/00032710802507927WOS:000263553400013Acesso restrito