Belintani, P.Gaspar, J. O.Targon, MLPNMachado, M. A.2014-05-202014-05-202002-06-01Journal of Phytopathology. Hoboken: Wiley-blackwell, v. 150, n. 6, p. 330-333, 2002.0931-1785http://hdl.handle.net/11449/22309Some molecular properties are described of Cole latent virus (CoLV), hitherto designated a tentative species of the Carlavirus genus. CoLV genomic RNA (Ribonucleic acid) of 8.3 Kb is polyadenylated. Two unencapsidated polyadenylated subgenomic RNAs (2.6 and 1.3 Kb) and three double-stranded RNAs (dsRNAs) (8.3, 2.6 and 1.3 Kbp), which are twice the size of the genomic and subgenomics ssRNAs, are produced in CoLV-infected plants, two additional dsRNAs (7.2 and 6.3 Kbp) were also detected plant extracts. By using a Carlavirus specific primer and a CoLV cDNA, a-3'-terminus fragment of 116 bp was amplified; it had homology with the carlaviruses Potato virus M (62%)., Hop latent virus (37%) and Blueberry scorch virus (36%) but no significant homology with 11 other carlaviruses. These results support the classification of CoLV as a distinct species of the Carlavirus genus.330-333engBrassica oleracea var. acephalapolyadenylationgenomic and subgenomic RNAscarlavirusBrasilArtigo10.1046/j.1439-0434.2002.00757.xWOS:000176354300005Acesso restrito