da Costa, M.Ximenes, Valdecir Farias [UNESP]Brunetti, Iguatemy Lourenço [UNESP]Falcao, R. P.Fonseca, Luiz Marcos da [UNESP]2014-05-202014-05-202004-01-01Hematology Journal. London: Nature Publishing Group, v. 5, n. 6, p. 496-499, 2004.1466-4860http://hdl.handle.net/11449/7563Introduction: In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).Methods: the frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.Results: All cases of AML-M7 and AML-M0 exhibited integrated light emission greater than 73 (10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-M0 showed results above the Cutoff point.Conclusion: This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-M1 cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.496-499engacute myeloid leukemia (AML)acute lymphoblastic leukemia (ALL)chemiluminescenceluminolp-iodophenolmyeloperoxidase (MPO)platelet peroxidase (PPO)Artigo10.1038/sj.thj.6200562WOS:000227379500007Acesso restrito4419635633356792