Salina, Margarete Aparecida [UNESP]Shikanai-Yasuda, Maria AparecidaMendes, Rinaldo Poncio [UNESP]Barraviera, Benedito [UNESP]Mendes-Giannini, Maria José Soares [UNESP]2014-05-272014-05-271998-06-01Journal of Clinical Microbiology, v. 36, n. 6, p. 1723-1728, 1998.0095-1137http://hdl.handle.net/11449/65454For the diagnosis and follow-up of paracoccidioidomycosis patients undergoing therapy, we evaluated two methods (immunoblotting and competition enzyme immunoassay) for the detection of circulating antigen in urine samples. A complex pattern of reactivity was observed in the immunoblot test. Bands of 70 and 43 kDa were detected more often in urine samples from patients before treatment. The immunoblot method detected gp43 and gp70 separately or concurrently in 11 (91.7%) of 12 patients, whereas the competition enzyme immunoassay detected antigenuria in 9 (75%) of 12 patients. Both tests appeared to be highly specific (100%), considering that neither fraction detectable by immunoblotting was present in urine samples from the control group. gp43 remained present in the urine samples collected during the treatment period, with a significant decrease in reactivity in samples collected during clinical recovery and increased reactivity in samples collected during relapses. Reactivity of some bands was also detected in urine specimens from patients with 'apparent cure.' The detection of Paracoccidioides brasiliensis antigens in urine appears to be a promising method for diagnosing infection, for evaluating the efficacy of treatment, and for detecting relapse.1723-1728engantigen detectionclinical articlediagnostic valueenzyme immunoassayhumanimmunoblottingparacoccidioides brasiliensispriority journalrecurrence riskreinfectionsouth american blastomycosisurinalysisAntifungal AgentsAntigens, FungalHumansImmunoblottingImmunoenzyme TechniquesParacoccidioidesParacoccidioidomycosisRecurrenceSensitivity and SpecificityParacoccidioides brasiliensisDetection of circulating Paracoccidioides brasiliensis antigen in urine of paracoccidioidomycosis patients before and during treatmentArtigoWOS:000073641300044Acesso aberto2-s2.0-00319685772-s2.0-0031968577.pdf0000-0002-8059-0826