Santos, Adriano [UNESP]Piccoli, Julia P. [UNESP]Filho, Norival A.s.Cilli, Eduardo Maffud [UNESP]2015-05-152015-05-152014Biosensors & Bioelectronics, v. 68, p. 281-287, 2014.0956-5663http://hdl.handle.net/11449/123463Early detection assays play a key role in the successful treatment of most diseases. Redox capacitive biosensors were recently introduced as a potential electroanalytical assay platform for point-of-care applications but alternative surfaces (besides a mixed layer containing ferrocene and antibody receptive component) for recruiting important clinical biomarkers are still needed. Aiming to develop alternative receptive surfaces for this novel electrochemical biosensing platform, we synthesized a ferrocene redoxtagged peptide capable of self-assembly into metallic interfaces, a potentially useful biological surface functionalization for bedside diagnostic assays. As a proof of concept we used C-reactive protein (CRP), as a model biomarker, and compared the obtained results to those of previously reported capacitive assays. The redox-tagged peptide approach shows a limit of detection of 0.8 nmol L 1 (same as 94 ng mL 1 ) and a linear range (R2 ∼98%) with the logarithm of the concentration of the analyte comprising 0.5–10.0 nmol L 1 , within a clinical relevant range for CRP.281-287porElectrochemical capacitance spectroscopyFerrocene-tagged peptideLabel-free redox capacitance biosensingBinding affinity constantArtigo10.1016/j.bios.2014.12.059Acesso restrito94243467624604160000-0002-4767-0904