Ibelli, A. M. G.Nakata, L. C.Andreo, R. [UNESP]Coutinho, L. L.Oliveira, M. C. S.Amarante, Alessandro Francisco Talamini do [UNESP]Furlong, J.Zaros, L. G.Regitano, L. C. A.2014-05-202014-05-202011-03-10Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 176, n. 2-3, p. 195-200, 2011.0304-4017http://hdl.handle.net/11449/18979Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naive Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei 13 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-gamma, MCP-1, lysozyme, pepsinogen and TNF-alpha genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-alpha were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-alpha was down-regulated (p < 0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p > 0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. (C) 2010 Elsevier B.V. All rights reserved.195-200engCytokinesBeef cattleHaemonchus placeiBos indicusGastrointestinal parasitesArtigo10.1016/j.vetpar.2010.11.013WOS:000289221400016Acesso abertoWOS000289221400016.pdf2677231663329706