Sakane, K. K.Monteiro, C. J.Silva, W.Silva, A. R.Santos, P. M.Lima, K. F.Moraes, K. C. M. [UNESP]2014-12-032014-12-032014-01-01Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 47, n. 1, p. 50-59, 2014.0100-879Xhttp://hdl.handle.net/11449/112703Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 mu M celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival.50-59engCellular and molecular analysesCell deathCyclooxygenase-2 inhibitorH9c2 cardiac cell lineArtigo10.1590/1414-431X20133028S0100-879X2013005003028WOS:000331907500007Acesso abertoS0100-879X2013005003028.pdf