Moraes, Marli L.Rodrigues Filho, Ubirajara P.Oliveira, Osvaldo N.Ferreira, Marystela [UNESP]2014-05-202014-05-202007-11-01Journal of Solid State Electrochemistry. New York: Springer, v. 11, n. 11, p. 1489-1495, 2007.1432-8488http://hdl.handle.net/11449/6805The layer-by-layer technique was exploited to immobilize the enzyme uricase onto indium tin oxide substrates coated with a layer of Prussian Blue. Uricase layers were alternated with either poly(ethylene imine) or poly(diallyidimethylammoniumchloride), and the resulting films were used as amperometric biosensors for uric acid. Biosensors with optimum perfomance had a limit of detection of 0.15 mu A mu mol 1(-1) cm(-2) with a linear response between 0.1 and 0.6 mu M of uric acid, which is sufficient for use in clinical tests. Bioactivity was preserved for weeks, and there was negligible influence from interferents, as detection was carried out at 0.0 V vs saturated calomel electrode.1489-1495englayer-by-layeramperometric biosensoruricasePrussian BlueArtigo10.1007/s10008-007-0314-4WOS:000251676900005Acesso restrito