Amaral, Mateus S.Loures, Carla C. A.Pedro, Guilherme A.Reis, Cristiano E. R.De Castro, Heizir E.Naves, Fabiano L.Silva, Messias B. [UNESP]Prata, Arnaldo M. R.2020-12-102020-12-102020-08-01Renewable Energy. Oxford: Pergamon-elsevier Science Ltd, v. 156, p. 591-601, 2020.0960-1481http://hdl.handle.net/11449/195443Biodiesel quality is dependent upon the fatty acids distribution of the raw material used in the trans/esterification reactions. While microalgae cells are regarded as potential oil suppliers for the biodiesel production chain, a common criticism is related to the low lipid productivities and often to a poor fatty acid distribution. In the present work we propose to submit microalgal cultivation to a second stage with nutritional stress by a simple addition of a 70% nitrate source depleted medium at the beginning of stationary phase, without harvesting cells, saving time and water. Chlorella minutissima was cultivated in a novel unconventional photobioreactor, named Internally Illuminated Integrated Photobioreactor (ILIPBR), to verify the efficiency of the proposed strategy. Biomass concentration, lipid content and volumetric lipids productivity increased 24.9%, 120.7% and 55.6%, respectively, from Stage 1 to Stage 2, when ILI-PBR was illuminated by constant blue LED and 0.5 vvm of aeration. The fatty acid profiles, evaluated in terms of LED illumination (white, red and blue), showed a predominance of palmitic acid in all cases (59.49%, 57.28% and 46.51%, respectively), which allowed to enhance some biodiesel properties such as cetane number, cold filter plugging point, density and kinematic viscosity, according to international specifications. (C) 2020 Elsevier Ltd. All rights reserved.591-601engChlorella minutissimaTwo-stage cultivationMicroalgaeBioreactorFatty acidBiodieselArtigo10.1016/j.renene.2020.04.084WOS:000540378500049