Artigos - Materiais Odontológicos e Prótese - FOAR

URI Permanente para esta coleção

https://hdl.handle.net/11449/16351

Navegar

Agora exibindo 1 - 20 de 926

Zerumbone Disturbs the Extracellular Matrix of Fluconazole-Resistant Candida albicans Biofilms
(2023-05-01) Abreu-Pereira, César Augusto [UNESP]; Gorayb-Pereira, Ana Luiza [UNESP]; Menezes Noveletto, João Vinícius [UNESP]; Jordão, Cláudia Carolina [UNESP]; Pavarina, Ana Cláudia [UNESP]; Universidade Estadual Paulista (UNESP)
This study assessed the effect of zerumbone (ZER) against fluconazole-resistant (CaR) and -susceptible Candida albicans (CaS) biofilms and verified the influence of ZER on extracellular matrix components. Initially, to determine the treatment conditions, the minimum inhibitory concentration (MIC), the minimum fungicidal concentration (MFC) and the survival curve were evaluated. Biofilms were formed for 48 h and exposed to ZER at concentrations of 128 and 256 µg/mL for 5, 10 and 20 min (n = 12). One group of biofilms did not receive the treatment in order to monitor the effects. The biofilms were evaluated to determine the microbial population (CFU/mL), and the extracellular matrix components (water-soluble polysaccharides (WSP), alkali-soluble polysaccharides (ASPs), proteins and extracellular DNA (eDNA), as well as the biomass (total and insoluble) were quantified. The MIC value of ZER for CaS was 256 μg/mL, and for CaR, it was 64 μg/mL. The survival curve and the MFC value coincided for CaS (256 μg/mL) and CaR (128 μg/mL). ZER reduced the cellular viability by 38.51% for CaS and by 36.99% for CaR. ZER at 256 µg/mL also reduced the total biomass (57%), insoluble biomass (45%), WSP (65%), proteins (18%) and eDNA (78%) of CaS biofilms. In addition, a reduction in insoluble biomass (13%), proteins (18%), WSP (65%), ASP (10%) and eDNA (23%) was also observed in the CaR biofilms. ZER was effective against fluconazole-resistant and -susceptible C. albicans biofilms and disturbed the extracellular matrix.
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity
(2023-01-01) Trigo-Gutierrez, Jeffersson Krishan [UNESP]; Calori, Italo Rodrigo; de Oliveira Bárbara, Geovana [UNESP]; Pavarina, Ana Claudia [UNESP]; Gonçalves, Renato Sonchini; Caetano, Wilker; Tedesco, Antonio Claudio; Mima, Ewerton Garcia de Oliveira [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); State University of Maringá
Nanocarriers have been successfully used to solubilize, deliver, and increase the bioavailability of curcumin (CUR), but slow CUR release rates hinder its use as a topical photosensitizer in antimicrobial photodynamic therapy. A photo-responsive polymer (PRP) was designed for the light-triggered release of CUR with an effective light activation-dependent antimicrobial response. The characterization of the PRP was compared with non-responsive micelles comprising Pluronics™ P123 and F127. According to the findings, the PRP formed photo-responsive micelles in the nanometric scale (< 100 nm) with a lower critical micelle concentration (3.74 × 10−4 M−1, 5.8 × 10−4 M−1, and 7.2 × 10−6 M−1 for PRP, F127, P123, respectively, at 25°C) and higher entrapment efficiency of CUR (88.7, 77.2, and 72.3% for PRP, F127, and P123 micelles, respectively) than the pluronics evaluated. The PRP provided enhanced protection of CUR compared to P123 micelles, as demonstrated in fluorescence quenching studies. The light-triggered release of CUR from PRP occurred with UV light irradiation (at 355 nm and 25 mW cm−2) and a cumulative release of 88.34% of CUR within 1 h compared to 80% from pluronics after 36 h. In vitro studies showed that CUR-loaded PRP was non-toxic to mammal cell, showed inactivation of the pathogenic microorganisms Candida albicans, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus, and decreased biofilm biomass when associated with blue light (455 nm, 33.84 J/cm2). The findings show that the CUR-loaded PRP micelle is a viable option for antimicrobial activity.
Bacterial response to Ti-35Nb-7Zr-5Ta alloy incorporated with calcium, phosphate and magnesium
(2023-05-01) dos Reis, Bárbara Araújo [UNESP]; Da Ponte Leguizamón, Natalia [UNESP]; Del Rey, Yumi Chokyu; Fernandes, Leandro [UNESP]; do Nascimento, Cássio [UNESP]; Vaz, Luis Geraldo [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
High implant survival rates have been achieved in recent decades due to continual modifications in implant design and surface topography, however there is still an ongoing quest to control peri-implant bone loss. The objective of this work was to develop Ti-35Nb-7Zr-5Ta (TNZT) alloys, perform physicochemical and morphological characterization of their surface modified by electrolytic oxidative plasma technique with ions related to osseointegration and lastly evaluate bacterial colonization in vitro. Three groups were evaluated: C group (polished TNZT), CaP group (sodium β glycerophosphate + calcium acetate) and Mg group (magnesium acetate). Before and after anodizing the surfaces, physicochemical and morphological analyses were performed: scanning electron microscopy with field emission gun (FEG-SEM), energy dispersion spectroscopy (EDS), X-ray diffraction (DRX), wettability (goniometer) and roughness (rugometer). Controlled and treated specimens were contaminated with unstimulated saliva collected from 10 healthy volunteers. Then, biofilm samples were collected and up to 35 microbial species, including commensal and pathogenic microorganisms, were identified and quantified by the Checkerboard DNA-DNA Hybridization method. The CaP group modified the surface morphology in the form of pores, while the Mg group modified it in the form of flakes. The contact angle was significantly smaller in the CaP group. The average roughness was higher in the CaP and Mg groups. A smaller total amount of bacteria was identified in the Mg group and relevant differences were found in the microbial profile associated with different surface treatments. Therefore, considering the microbiological profile and for the prevention of peri-implantitis, the Mg group presented more satisfactory and encouraging results for the manufacture of dental implants Graphical Abstract: [Figure not available: see fulltext.].
Reliability and lifetime of lithium disilicate, 3Y-TZP, and 5Y-TZP zirconia crowns with different occlusal thicknesses
(2023-01-01) Adabo, Gelson Luis [UNESP]; Longhini, Diogo [UNESP]; Baldochi, Mariana Ribeiro [UNESP]; Bergamo, Edmara Tatiely Pedroso; Bonfante, Estevam Augusto; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
Objectives: To assess the probability of survival of crowns made with a 3Y-TZP, a 5Y-TZP, and a lithium disilicate. Materials and methods: CAD-CAM premolar crowns with occlusal thicknesses of 1.0 mm or 1.5 mm and cemented on a metal dye. Step-stress accelerated life testing (SSALT) was performed to access the use level probability Weibull curves and reliability were calculated for the completion of a mission of 100,000 cycles at 300 to 1200 N. Results: All ceramics showed a high probability of survival (87–99%) at 300 N, irrespective of thickness. 3Y-TZP shows no significant decrease in the probability of survival up to 1200 N (83–96%). Lithium disilicate presented lower reliability than zirconia under the 600 N mission. 5Y-TZP showed lower reliability than 3Y-TZP at 1200 N. There was no significant difference in the Weibull modulus (3.23–7.12). 3Y-TZP had the highest characteristic strength (2483–2972 N), followed by 5Y-TZP (1512–1547 N) and lithium disilicate (971–1154 N). Conclusion: Zirconia ceramics have a high probability of survival (up to 900 N-load), while lithium disilicate survives up to 300 N load only, irrespective of the thickness (1.0 mm or 1.5 mm). Clinical relevance: The probability of survival of posterior crowns made with zirconia ceramics resists extreme loads while glass ceramics resist normal chewing loads. In addition, crowns with thinner occlusal face showed sufficient mechanical behavior.
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
(2023-01-01) Dias, Luana Mendonça [UNESP]; Klein, Marlise Inêz [UNESP]; Ferrisse, Túlio Morandin [UNESP]; Medeiros, Karine Sousa [UNESP]; Jordão, Cláudia Carolina [UNESP]; Bellini, Amanda [UNESP]; Pavarina, Ana Claudia [UNESP]; Universidade Estadual Paulista (UNESP)
This study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine® (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L−; C+L−), and LED (P−L+; C−L+). Control groups were used (P−L−; C−L−). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed ~1.40 log10 increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ.
Synthesis of Organic Semiconductor Nanoparticles with Different Conformations Using the Nanoprecipitation Method
(2022-12-01) Yoshioka, Nathalia A.; Faraco, Thales A.; Barud, Hernane S.; Ribeiro, Sidney J. L. [UNESP]; Cremona, Marco; Fragneaud, Benjamin; Maciel, Indhira O.; Quirino, Welber G.; Legnani, Cristiano; Universidade Federal de Juiz de Fora (UFJF); Universidade de Araraquara (UNIARA); Universidade Estadual Paulista (UNESP); Pontifícia Universidade Católica do Rio de Janeiro (PUC-Rio)
In recent years, nanoparticulate materials have aroused interest in the field of organic electronics due to their high versatility which increases the efficiency of devices. In this work, four different stable conformations based on the organic semiconductors P3HT and PC71BM were synthesized using the nanoprecipitation method, including blend and core-shell nanoparticles. All nanoparticles were obtained free of surfactants and in aqueous suspensions following the line of ecologically correct routes. The structural and optoelectronic properties of the nanoparticles were investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM), UV-visible absorption spectroscopy and UV-visible photoluminescence (PL). Even in aqueous media, the blend and core-shell nanoparticles exhibited a greater light absorption capacity, and these conformations proved to be effective in the process of dissociation of excitons that occurs at the P3HT donor/PC71BM acceptor interface. With all these characteristics and allied to the fact that the nanoparticles are surfactant-free aqueous suspensions, this work paves the way for the use of these colloids as a photoactive layer of organic photovoltaic devices that interface with biological systems.
Dental Whitening: Self-Referred Needs versus Professional Indication
(2022-01-24) Cavalcanti, Jéssica de Oliveira Sotero; Neto, Inaldo Nogueira de Oliveira; Dias, Marlon Ferreira; Lins-Filho, Paulo Cardoso; Guimarães, Renata Pedrosa; Universidade Federal de Pernambuco (UFPE); Faculdade de Odontologia de Araraquara
Objective: To analyze the self-reported need of patients compared to professional indications for tooth whitening. Material and Methods: Initially, 58 undergraduate students responded to a form that highlighted the question: Do you think you need to have your teeth whitened? Among those who answered positively to the previous question, ten individuals were photographed with their smiles. In addition, they were asked to point out, on the Vita 3D-Master scale, which color they believed their teeth had, a value that was compared to the actual color obtained by a spectrophotometer. Finally, the photographs were presented to dentists, who were asked about the indication or not of the whitening treatment. Results: Most interviewees (63.8%) self-reported the need for whitening, as well as there was a greater incidence of a positive indication among professionals (53.9%). Pearson's Chi-square test revealed a relationship between patient gender and the training course on the desire to have teeth whitened. Among the professionals, the specialty, as well as time since graduation, interfered in the indication for whitening. Conclusion: Professionals and patients share the aesthetic ideal directly related to light teeth; most patients self-perceive the color of their teeth darker than it actually is; the opinion about the color of the teeth has an extremely subjective character and varies greatly from one professional to another.
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
(2023-01-01) Pansani, Taisa Nogueira [UNESP]; Basso, Fernanda Gonçalves; Cardoso, Laís Medeiros [UNESP]; de Souza Costa, Carlos Alberto [UNESP]; Universidade Estadual Paulista (UNESP); UNAERP
Objective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α). Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL-6, IL-8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results: Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions: EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus.
Hydrogen peroxide enhances the efficacy of photodynamic therapy against Candida albicans biofilms
(2023-01-01) Viana de Sousa, Tábata [UNESP]; Carolina Jordão, Cláudia [UNESP]; Augusto Abreu-Pereira, César [UNESP]; Gorayb Pereira, Ana Luiza [UNESP]; Barbugli, Paula Aboud [UNESP]; Klein, Marlise Inêz; Pavarina, Ana Claudia [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Estadual de Campinas (UNICAMP)
The present study aimed to evaluate the effectiveness of hydrogen peroxide (H2O2) combined with antimicrobial photodynamic therapy (aPDT) on biofilms formed by Candida albicans strains which are either susceptible to or resistant to fluconazole. Biofilms were grown and treated with H2O2, followed by the application of Photodithazine® (P) and red light-emitting diode (LED) (L) either separately or combined (n = 12). After the treatment, biofilms were evaluated by estimating colony-forming unit ml−1, extracellular matrix components [water -soluble and -insoluble polysaccharides, proteins, extracellular DNA (eDNA)], biomass (total and insoluble dry-weight), and protein concentration. Biofilms formed by both strains presented a significant reduction in cell viability, biomass, extracellular matrix components (both types of polysaccharides, eDNA), and proteins (in the soluble and insoluble portion of biofilms) compared to the control. Microscopy images of the biofilms after treatments showed disarticulation of the matrix and scattered fungal cells. The application of H2O2 can disturb the organization of the extracellular matrix, and its association with aPDT potentiated the effect of the treatment.
Physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic repair material
(2023-01-01) Campi, Lívia Bueno [UNESP]; Rodrigues, Elisandra Márcia [UNESP]; Torres, Fernanda Ferrari Esteves [UNESP]; Reis, José Maurício Dos Santos Nunes [UNESP]; Guerreiro-Tanomaru, Juliane Maria [UNESP]; Tanomaru-Filho, Mário [UNESP]; Universidade Estadual Paulista (UNESP)
The aim of this study was to evaluate the physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic material, Bio-C Repair (Angelus), in comparison with White MTA (Angelus) and Biodentine (Septodont). The physicochemical properties of setting time, radiopacity, pH, solubility, dimensional and volumetric changes were evaluated. Biocompatibility and bioactivity were assessed in Saos-2 osteoblast cell cultures by the MTT assay 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), Neutral Red (NR), Alizarin Red (ARS), and cell migration tests. Statistical analysis was performed by ANOVA, Tukey or Bonferroni tests (α = 0.05). Bio-C Repair had the longest setting time (p < 0.05), but radiopacity and solubility were accordance with the ISO 6876/2012 standards, besides linear expansion. Bio-C Repair and MTA had similar volumetric change (p > 0.05); lower than Biodentine (p < 0.05). All the materials evaluated had an alkaline pH. Bio-C Repair was cytocompatible and promoted mineralized nodule deposition in 21 days and cell migration in 3 days. In conclusion, Bio-C Repair had adequate radiopacity above 3mm Al, solubility less than 3%, dimensional expansion, and low volumetric change. In addition, Bio-C Repair promoted an alkaline pH and presented bioactivity and biocompatibility similar to MTA and Biodentine, showing potential for use as a repair material.
Effect of immersion in disinfectants on the color stability of denture base resins and artificial teeth obtained by 3D printing
(2023-01-01) Coelho, Sabrina R. G. [UNESP]; da Silva, Marcela D. D. [UNESP]; Nunes, Thais S. B. S. [UNESP]; Viotto, Hamile E. C. [UNESP]; Marin, Danny O. M.; Pero, Ana C. [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de Santa Catarina (UFSC)
Purpose: To evaluate the effect of immersion in disinfecting solutions on the color stability of denture base resins and artificial teeth obtained by 3D printing. Materials and methods: Forty discs (15 × 3 mm) were obtained for each group: Lucitone 550 and Cosmos Denture 3D (denture base resins), Duralay and Cosmos TEMP 3D (artificial teeth resins). The discs were immersed in disinfectant solutions: Corega Tabs, 2% chlorhexidine digluconate, 0.25% sodium hypochlorite, and distilled water. Color measurements were obtained with a spectrophotometer before immersion in disinfectants and after the simulated periods of 6 and 12 months. Data (ΔE00) were submitted to mixed three-way ANOVA and Bonferroni post-test. Results: For denture base resins, Cosmos Denture 3D showed greater color change regardless of the solution and immersion time. The immersion time of 6 months influenced the color change of the denture base resins regardless of the disinfectant solution. For the artificial teeth resins, the immersion time of 12 months showed a significant color change when compared to 6 months. Cosmos TEMP 3D showed greater color change for all solutions, except for 0.25% sodium hypochlorite. Duralay resin showed greater color change in 2% chlorhexidine, regardless of immersion time. Conclusions: For denture base resins, the immersion time significantly changed the color regardless of the solution. For artificial teeth resins, Cosmos TEMP 3D showed greater color changes in all solutions when compared to Duralay, except for 0.25% sodium hypochlorite. Chlorhexidine digluconate significantly changed the color of Duralay.
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes
(2023-03-03) Miranda, Luis Fernando B.; Lima, Carolina V.; Pagin, Rafaela; Costa, Raphael C.; Pereira, Marta Maria A. [UNESP]; de Avila, Erica D. [UNESP]; Bertolini, Martinna; Retamal-Valdes, Belén; Shibli, Jamil A.; Feres, Magda; Barão, Valentim A. R.; Souza, João Gabriel S.; Universidade Estadual de Campinas (UNICAMP); Universidade Federal do Paraná (UFPR); Guarulhos University (UnG); Universidade Estadual Paulista (UNESP); University of Pittsburgh; Dental Science School (Faculdade de Ciências Odontológicas - FCO)
The use of saliva as a protein source prior to microbiological and biological assays requires previous processing. However, the effect of these processing methods on the proteomic profile of saliva has not been tested. Stimulated human saliva was collected from eight healthy volunteers. Non-processed saliva was compared with 0.22 μm filtered, 0.45 μm filtered, and pasteurized saliva, by liquid chromatography-mass spectrometry. Data are available via ProteomeXchange with identifier PXD039248. The effect of processed saliva on microbial adhesion was tested using bacterial and fungus species and in biological cell behavior using HaCaT immortalized human keratinocytes. Two hundred and seventy-eight proteins were identified in non-processed saliva, of which 54 proteins (≈19%) were exclusive. Saliva processing reduced identified proteins to 222 (≈80%) for the 0.22 μm group, 219 (≈79%) for the 0.45 μm group, and 201 (≈72%) for the pasteurized saliva, compared to non-processed saliva. The proteomic profile showed similar molecular functions and biological processes. The different saliva processing methods did not alter microbial adhesion (ANOVA, p > 0.05). Interestingly, pasteurized saliva reduced keratinocyte cell viability. Saliva processing methods tested reduced the proteomic profile diversity of saliva but maintained similar molecular functions and biological processes, not interfering with microbial adhesion and cell viability, except for pasteurization, which reduced cell viability.
Paracoccidioidomycosis: An Update from the Environment to Clinics
(2022-01-01) de Oliveira, Haroldo Cesar; de Paula e Silva, Ana Carolina Alves [UNESP]; Marcos, Caroline Maria [UNESP]; de Fátima da Silva, Julhiany [UNESP]; Scorzoni, Liliana [UNESP]; Assato, Patricia Akemi [UNESP]; Rossi, Suélen Andreia; Fundação Oswaldo Cruz (Fiocruz-PR); Universidade Estadual Paulista (UNESP); Guarulhos University (UNG); Universidade de São Paulo (USP)
Paracoccidioidomycosis is an endemic mycosis of great importance in Latin America caused by dimorphic fungi of the genus Paracoccidioides. Recent advances in the study of this mycosis reveal the Paracoccidioides genus as fungi that are difficult to treat and diagnose, with a great capacity to adapt and evade the environment of host cells, causing an invasive disease that is neglected in areas where it is endemic. This chapter aims to provide an overview of the fungi of this genus and the disease, bringing historical aspects and also the most recent advances in the study of this important mycosis, which has a great impact on public health in many countries in Latin America. This chapter will address issues such as the diversity of species of the Paracoccidioides genus, the thermal dimorphism, that allows the fungus to survive in the environment and the host, and its impact on the infectious process, virulence factors, and their relationship with the evasion capacity of the host immune system, as well as aspects associated with the disease such as clinical forms, pathogenesis, epidemiology, diagnosis and treatment.
Mapping the trends and impact of research collaboration between countries in oral implantology publications: A bibliometric analysis from 1999 to 2019
(2022-01-01) Dini, Caroline; Pereira, Marta Maria Alves [UNESP]; Souza, João Gabriel Silva; de Avila, Erica Dorigatti [UNESP]; Barão, Valentim Adelino R.; Universidade Estadual de Campinas (UNICAMP); Universidade Estadual Paulista (UNESP); Guarulhos University (UnG)
Statement of problem: Scientific collaboration provides a suitable strategy for enhancing the exchange of knowledge and technological development. However, the impact of collaboration in oral implantology research between countries and how it has been influenced by the income status of the country has not been previously evaluated. Purpose: The purpose of this bibliometric analysis was to evaluate how collaboration between countries affected oral implant publications and whether patterns of collaboration differ depending on the country's income. Material and methods: Articles were retrieved from 7 well-established journals whose scope included oral implantology at 5 time points (1999, 2004, 2009, 2014, and 2019). Data were extracted, and descriptive, bivariate, and multivariate logistic regression analyses were performed (α=.05). Results: A total of 1944 articles were included; of which, 27.5% presented collaboration between countries. In 2009, 2014, and 2019, collaboration between countries was more likely to occur than at previous time points (P≤.005). Corresponding authors with a higher h-index (P<.05) and authors from Europe and North America (P<.001) were more likely to establish international connections. The possibility of collaboration between countries was higher for animal studies (P<.001) and for articles published by Clinical Oral Implants Research (P=.026). Collaborations between high-income and upper-middle– or lower-middle–income countries were more likely to happen in 2014 and 2019 (P<.05), as well as when the number of authors was higher (P=.015), compared with collaboration between high-income countries. With regard to the continent, European articles were less likely to have collaborations with upper-middle– or lower-middle–income countries (P<.001). Conclusions: The findings revealed that some parameters related to the study, authors, countries, and journals were statistically associated with the presence of collaboration between countries. However, the bibliometric parameters showed different trends when countries of different incomes established collaboration.
Effect of repair methods and materials on the flexural strength of 3D-printed denture base resin
(2022-01-01) do Carmo Viotto, Hamile Emanuella [UNESP]; Silva, Marcela Dantas Dias [UNESP]; Nunes, Thaís Soares Bezerra Santos [UNESP]; Coelho, Sabrina Romão Gonçalves [UNESP]; Pero, Ana Carolina [UNESP]; Universidade Estadual Paulista (UNESP)
PURPOSE. The aim of this study was to evaluate the flexural strength of a 3D-printed denture base resin (Cosmos Denture), after different immediate repair techniques with surface treatments and thermocycling. MATERIALS AND METHODS. Rectangular 3D-printed denture base resin (Cosmos Denture) specimens (N = 130) were thermocycled (5,000 cycles, 5°C and 55°C) before and after the different repair techniques (n = 10 per group) using an autopolymerized acrylic resin (Jet, J) or a hard relining resin (Soft Confort, SC), and different surface treatments: Jet resin monomer for 180 s (MMA), blasting with aluminum oxide (JAT) or erbium: yttrium-aluminum-garnet laser (L). The control group were intact specimens. A three-point flexural strength test was performed, and data (MPa) were analyzed by ANOVA and Games-Howell post hoc test (α = 0.05). Each failure was observed and classified through stereomicroscope images and the surface treatments were viewed by scanning electron microscope (SEM). RESULTS. Control group showed the highest mean of flexural strength, statistically different from the other groups (P <.001), followed by MMA+J group. The groups with L treatment were statistically similar to the MMA groups (P >.05). The JAT+J group was better than the SC and JAT+SC groups (P <.05), but similar to the other groups (P >.05). Adhesive failures were most observed in JAT groups, especially when repaired with SC. The SEM images showed surface changes for all treatments, except JAT alone. CONCLUSION. Denture bases fabricated with 3D-printed resin should be preferably repaired with MMA+J. SC and JAT+SC showed the worst results. Blasting impaired the adhesion of the SC resin.
Efficacy of Antimicrobial Photodynamic Therapy Mediated by Photosensitizers Conjugated with Inorganic Nanoparticles: Systematic Review and Meta-Analysis
(2022-10-01) Ferrisse, Túlio Morandin [UNESP]; Dias, Luana Mendonça [UNESP]; de Oliveira, Analú Barros [UNESP]; Jordão, Cláudia Carolina [UNESP]; Mima, Ewerton Garcia de Oliveira [UNESP]; Pavarina, Ana Claudia [UNESP]; Universidade Estadual Paulista (UNESP)
Antimicrobial photodynamic therapy (aPDT) is a method that does not seem to promote antimicrobial resistance. Photosensitizers (PS) conjugated with inorganic nanoparticles for the drug-delivery system have the purpose of enhancing the efficacy of aPDT. The present study was to perform a systematic review and meta-analysis of the efficacy of aPDT mediated by PS conjugated with inorganic nanoparticles. The PubMed, Scopus, Web of Science, Science Direct, Cochrane Library, SciELO, and Lilacs databases were searched. OHAT Rob toll was used to assess the risk of bias. A random effect model with an odds ratio (OR) and effect measure was used. Fourteen articles were able to be included in the present review. The most frequent microorganisms evaluated were Staphylococcus aureus and Escherichia coli, and metallic and silica nanoparticles were the most common drug-delivery systems associated with PS. Articles showed biases related to blinding. Significant results were found in aPDT mediated by PS conjugated with inorganic nanoparticles for overall reduction of microorganism cultured in suspension (OR = 0.19 [0.07; 0.67]/p-value = 0.0019), E. coli (OR = 0.08 [0.01; 0.52]/p-value = 0.0081), and for Gram-negative bacteria (OR = 0.12 [0.02; 0.56/p-value = 0.0071). This association approach significantly improved the efficacy in the reduction of microbial cells. However, additional blinding studies evaluating the efficacy of this therapy over microorganisms cultured in biofilm are required.
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
(2022-10-28) Dos Santos, Danilo M.; Dias, Luana M. [UNESP]; Surur, Amanda K. [UNESP]; De Moraes, Daniel A.; Pavarina, Ana C. [UNESP]; Fontana, Carla R. [UNESP]; Correa, Daniel S.; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA); Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
Oxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2NPs) and manganese dioxide nanosheets (MnO2NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2NSs were applied as a nanozyme to catalyze the decomposition of H2O2to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2NPs and MnO2NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2NP and MnO2NS were incorporated into the fiber's structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration.
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping
(2022-11-01) Leite, Maria Luísa; Anselmi, Caroline [UNESP]; Soares, Igor Paulino Mendes [UNESP]; Manso, Adriana Pigozzo; Hebling, Josimeri [UNESP]; Carvalho, Ricardo Marins; de Souza Costa, Carlos Alberto [UNESP]; The University of British Columbia; Universidade Estadual Paulista (UNESP)
Objectives: This study aimed to develop and characterize different formulations of porous chitosan scaffolds (SCH) associated with calcium silicate (CaSi) and evaluate their chemotactic and bioactive potential on human dental pulp cells (hDPCs). Methods: Different concentrations of CaSi suspensions (0.5%, 1.0%, and 2.0%, w/v) were incorporated (1:5; v/v) /or not, into 2% chitosan solution, giving rise to the following groups: SCH (control); SCH+ 0.5CaSi; SCH+ 1.0CaSi; SCH+ 2.0 CaSi. The resulting solutions were submitted to thermally induced phase separation followed by freeze-drying procedures to obtain porous scaffolds. The topography, pH, and calcium release kinetics of the scaffolds were assessed. Next, the study evaluated the influence of these scaffolds on cell migration (MG), viability (VB), proliferation (PL), adhesion and spreading (A&S), and on total protein synthesis (TP), alkaline phosphatase (ALP) activity, mineralized matrix deposition (MMD), and gene expression (GE) of odontogenic differentiation markers (ALP, DSPP, and DMP-1). The data were analyzed with ANOVA complemented with the Tukey post-hoc test (α = 5%). Results: Incorporation of the CaSi suspension into the chitosan scaffold formulation increased pore diameter when compared with control. Increased amounts of CaSi in the CH scaffold resulted in higher pH values and Ca release. In Groups SCH+ 1.0CaSi and SCH+ 2.0CaSi, increased VB, PF, A&S, GE of DSPP/DMP-1 and MMD values were shown. However, Group SCH+ 2.0CaSi was the only formulation capable of enhancing MG and showed the highest increase in TP, MMD, and GE of DMP-1 and DSPP values. Significance: SCH+ 2.0CaSi formulation had the highest chemotactic and bioactive potential on hDPCs and may be considered a potential biomaterial for pulp-dentin complex regeneration.
Disclosing the Biocide Activity of α-Ag2−2xCuxWO4 (0 ≤ x ≤ 0.16) Solid Solutions
(2022-09-01) Pereira, Paula Fabiana dos Santos; De Foggi, Camila Cristina; Gouveia, Amanda Fernandes; Pinatti, Ivo Mateus; Cabral, Luís Antônio; Guillamon, Eva; Sorribes, Iván; San-Miguel, Miguel A.; Vergani, Carlos Eduardo [UNESP]; Simões, Alexandre Zirpoli [UNESP]; da Silva, Edison Z.; Cavalcante, Laécio Santos; Llusar, Rosa; Longo, Elson; Andrés, Juan; Universidade Federal de São Carlos (UFSCar); University Jaume I (UJI); Federal University of Rio Grande do Sul; Universidade Estadual de Campinas (UNICAMP); Federal University of Maranhao; Universidade Estadual Paulista (UNESP); Universidade Estadual do Piauí
In this work, α-Ag2−2xCuxWO4 (0 ≤ x ≤ 0.16) solid solutions with enhanced antibacterial (against methicillin-resistant Staphylococcus aureus) and antifungal (against Candida albicans) activities are reported. A plethora of techniques (X-ray diffraction with Rietveld refinements, inductively coupled plasma atomic emission spectrometry, micro-Raman spectroscopy, attenuated total reflectance–Fourier transform infrared spectroscopy, field emission scanning electron microscopy, ultraviolet–visible spectroscopy, photoluminescence emissions, and X-ray photoelectron spectroscopy) were employed to characterize the as-synthetized samples and determine the local coordination geometry of Cu2+ cations at the orthorhombic lattice. To find a correlation between morphology and biocide activity, the experimental results were sustained by first-principles calculations at the density functional theory level to decipher the cluster coordinations and electronic properties of the exposed surfaces. Based on the analysis of the under-coordinated Ag and Cu clusters at the (010) and (101) exposed surfaces, we propose a mechanism to explain the biocide activity of these solid solutions.
Computer-Guided Implantology
(Intech Europe, 2011-01-01) Margonar, Rogerio; Correa, Cassia Bellotto [UNESP]; Roselino Ribeiro, Ana Lucia [UNESP]; Almeida, Erika Oliveira de [UNESP]; Marcantonio, Elcio [UNESP]; Vaz, Luis Geraldo [UNESP]; Turkyilmaz, I; Araraquara Univ Ctr UNIARA; Universidade Estadual Paulista (UNESP); NYU

Navegar

Submissões Recentes