Glândulas mandibulares de operárias de formigas Atta sexdens rubropilosa (Forel, 1908): morfologia e expressão gênica (Hymenoptera: Formicidae)
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Data
2006-06-21
Autores
Porfirio, Lorena Favaro Pavon [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
As glândulas mandibulares, dos insetos Hymenoptera, fazem parte do seu sistema salivar. São estruturas pares que se localizam uma em cada lado da cabeça, possuindo um ducto excretor abrindo na base da articulação da mandíbula, por onde eliminam a secreção produzida. São compostas por um reservatório associado às células secretoras, sendo que cada célula desemboca individualmente no reservatório através de canalículos. Embora as glândulas mandibulares estejam ligadas às peças bucais, utilizando seu produto glandular na ingestão e digestão de alimentos, essas glândulas evoluíram no sentido de se dissociarem da função digestiva para terem função comportamental. No presente estudo avaliou-se a morfo-histologia, citoquímica ultra-estrutural, dosagem e eletroforese de proteínas, além da expressão gênica das glândulas mandibulares das operárias mínimas, médias e soldados de formigas Atta sexdens rubropilosa, com o objetivo de fornecer informações sobre a dinâmica da produção, bem como a composição e origem da secreção dessas glândulas nas três castas desses insetos. A análise morfológica e ultra-estrutural evidenciou uma organização glandular saculiforme, constituída por uma porção secretora e um reservatório com um ducto excretor a ele conectado. A porção secretora é formada por células arranjadas em ácinos e o reservatório é constituído por um epitélio simples pavimentoso revestido pela íntima cuticular. Essas duas porções encontram-se interligadas por canalículos intra e extracitoplasmáticos. Tanto a histoquímica, quanto a citoquímica ultra-estrutural detectou lipídios, proteínas e polissacarídeos nas diferentes regiões das glândulas mandibulares nas três castas estudadas, inclusive em suas secreções, demonstrando...
The mandibular glands, in the Hymenoptera insects, make up part of the salivary system. These glands are paired structures that are located one on each side of the head, containing an excretory duct, at the base of the mandible articulation, from which the secretion produced is eliminated. They contain a reservoir associated with the secretory cells, being that each cell opens individually in the reservoir through the canaliculi. Even though the mandibular glands are connected to the bucal parts, using its glandular product in the ingestion and digestion of food, these glands have evolved in the sense of being disassociated with the digestive function to having a behavioural function. In the present study the morpho-histology, ultrastructural cytochemistry, protein dosage and electrophoresis were evaluated, as well as the gene expression of the mandibular glands of the minima, media workers and soldiers of ants Atta sexdens rubropilosa, with the objective of providing information about the production dynamics, as well as the composition and origin of the secretion of these glands in the three castes of these insects. The morphological and ultrastructural analysis described a glandular organization consisting of sacs, formed by a secretory portion and a reservoir with a connected excretory duct. The secretory portion is formed by cells arranged in alveoli and the reservoir is constituted of a simple pavimentous epithelium covered by the cuticular intima. These two portions are interconnected by intra- and extra-cytoplasmic canaliculi. The histochemistry, as well the ultrastructural cytochemistry, highlighted lipids, proteins and polysaccharides in different regions of the mandibular glands in the three castes studied demonstrating... (Complete abstract, click electronic address below)
The mandibular glands, in the Hymenoptera insects, make up part of the salivary system. These glands are paired structures that are located one on each side of the head, containing an excretory duct, at the base of the mandible articulation, from which the secretion produced is eliminated. They contain a reservoir associated with the secretory cells, being that each cell opens individually in the reservoir through the canaliculi. Even though the mandibular glands are connected to the bucal parts, using its glandular product in the ingestion and digestion of food, these glands have evolved in the sense of being disassociated with the digestive function to having a behavioural function. In the present study the morpho-histology, ultrastructural cytochemistry, protein dosage and electrophoresis were evaluated, as well as the gene expression of the mandibular glands of the minima, media workers and soldiers of ants Atta sexdens rubropilosa, with the objective of providing information about the production dynamics, as well as the composition and origin of the secretion of these glands in the three castes of these insects. The morphological and ultrastructural analysis described a glandular organization consisting of sacs, formed by a secretory portion and a reservoir with a connected excretory duct. The secretory portion is formed by cells arranged in alveoli and the reservoir is constituted of a simple pavimentous epithelium covered by the cuticular intima. These two portions are interconnected by intra- and extra-cytoplasmic canaliculi. The histochemistry, as well the ultrastructural cytochemistry, highlighted lipids, proteins and polysaccharides in different regions of the mandibular glands in the three castes studied demonstrating... (Complete abstract, click electronic address below)
Descrição
Palavras-chave
Formiga, Biologia molecular, Glândulas mandibulares, Morfologia, Ultra-estrutura, Mandibular glands, Ants, Atta sexdens rubropilosa, Molecular biology
Como citar
PORFIRIO, Lorena Favaro Pavon. Glândulas mandibulares de operárias de formigas Atta sexdens rubropilosa (Forel, 1908): morfologia e expressão gênica (Hymenoptera: Formicidae). 2006. viii, 128 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2006.