Efeito de diferentes tempos de deficiência estrogênica na perda óssea resultante da periodontite experimental em ratas
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Data
2007-06-20
Autores
Amadei, Susana Ungaro [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Este estudo avaliou os efeitos de diferentes tempos de deficiência estrogênica na perda óssea resultante da periodontite induzida em ratas. Foram utilizadas 80 ratas, divididas em dois grupos: ovariectomizado-OVZ (n=40) e cirurgia simulada SHAM (n=40) e subdivididas de acordo com o tempo de deficiência estrogênica (30, 60, 90 e 120 dias). Os animais foram submetidos à indução de periodontite com fio de algodão na cervical do primeiro molar inferior esquerdo e segundo molar superior direito. Tal ligadura foi mantida nos últimos 30 dias de experimento. Após o sacrifício, as mandíbulas e maxilas foram removidas para análises radiográfica, morfométrica e histométrica. Nos grupos com e sem ligadura, foram considerados variáveis o tempo e a deficiência estrogênica, aplicando-se ANOVA e teste Tukey (5%). A comparação intragrupo demonstrou que a ligadura induziu periodontite em todas as análises. Na análise radiográfica, com ou sem ligadura observou-se influência da deficiência hormonal na redução do suporte ósseo periodontal em todos os períodos, sendo os valores nos grupos OVZ inferiores aos SHAM. O fator tempo foi significativo ao analisarem-se os grupos com ligadura, sendo o período de 90 dias o que apresentou menor valor. Comparando-se os grupos sem ligadura nas análises morfométricas e histométricas, os efeitos da deficiência estrogênica e do tempo não foram significativos. Entretanto, entre os grupos com ligadura, aqueles de 90 dias apresentaram significativa perda óssea. Concluiu-se que a deficiência estrogênica, aos noventa dias, agravou a perda óssea resultante da periodontite em ratas.
This study evaluated the different times of estrogen defficiency effects on loss bone induced by experimental periodontitis in female rats. Eighty female rats were used and were divided into two groups: ovariectomized-OVZ (n=40) and simulated surgery SHAM (n=40) and subdivided according to the times of estrogen defficiency (30, 60, 90 and 120 days). The animals were submitted to induced periodontitis using cotton thread in the cervical of the first inferior molar and second superior molar in opposite sides. Such ligature was kept in the last 30 days of experiment. After the sacrifice, the mandibles and maxillas were removed for radiographic, morphometric and histometric analysis. In both groups, with and without ligatures, the time and the estrogen defficiency were considered variables and ANOVA and Tukey test were applied (5%). The comparison within groups demonstrated that the ligature induced periodontitis in all analysis. In the radiographic analysis, with or without ligature, the influence of the hormonal deficiency in the decrease of the periodontal osseous support in all periods was observed, being the values in the OVZ group lower than SHAM ones. The factor time was significant at the analysis of the ligature groups, and the groups studied within 90 days presented the lowest value. Comparing the groups without ligature in the morphometric and histometric analysis, the estrogen defficiency and time effects were not verified. However, among the ligature groups, those treated within 90 days showed significant bone loss. We can conclude that reaching 90 days of estrogen defficiency it was possible an adequate study of the periodontal disease in osteopenic rats; therefore, it is the ideal period to evaluate this relation.
This study evaluated the different times of estrogen defficiency effects on loss bone induced by experimental periodontitis in female rats. Eighty female rats were used and were divided into two groups: ovariectomized-OVZ (n=40) and simulated surgery SHAM (n=40) and subdivided according to the times of estrogen defficiency (30, 60, 90 and 120 days). The animals were submitted to induced periodontitis using cotton thread in the cervical of the first inferior molar and second superior molar in opposite sides. Such ligature was kept in the last 30 days of experiment. After the sacrifice, the mandibles and maxillas were removed for radiographic, morphometric and histometric analysis. In both groups, with and without ligatures, the time and the estrogen defficiency were considered variables and ANOVA and Tukey test were applied (5%). The comparison within groups demonstrated that the ligature induced periodontitis in all analysis. In the radiographic analysis, with or without ligature, the influence of the hormonal deficiency in the decrease of the periodontal osseous support in all periods was observed, being the values in the OVZ group lower than SHAM ones. The factor time was significant at the analysis of the ligature groups, and the groups studied within 90 days presented the lowest value. Comparing the groups without ligature in the morphometric and histometric analysis, the estrogen defficiency and time effects were not verified. However, among the ligature groups, those treated within 90 days showed significant bone loss. We can conclude that reaching 90 days of estrogen defficiency it was possible an adequate study of the periodontal disease in osteopenic rats; therefore, it is the ideal period to evaluate this relation.
Descrição
Palavras-chave
Osteoporose, Periodontite, Ovarictomia, Doenças periodontais, Perda óssea alveolar, Deficiência estrogênica, Osteoporosis, Periodontal diseases
Como citar
AMADEI, Susana Ungaro. Efeito de diferentes tempos de deficiência estrogênica na perda óssea resultante da periodontite experimental em ratas. 2007. 85 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de São José dos Campos, 2007.