Efeito do plasma rico em plaquetas associado ao enxerto autógeno na tíbia de coelhos: estudos histomorfométrico e biomecânico
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Data
2007-07-02
Autores
Monteiro, Adriana do Socorro Ferreira [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O propósito deste trabalho foi avaliar o efeito do plasma rico em plaquetas associados ou não ao enxerto ósseo autógeno no processo de reparação óssea em defeitos cirúrgicos confeccionados na tíbia de coelhos. Nesta pesquisa foram utilizados 25 coelhos adultos, nos quais foram realizados 2 defeitos em cada tíbia, divididos nos seguintes grupos de acordo com o tratamento: controle (C - defeito preenchido somente por coágulo sangüíneo), autógeno (A - defeito + enxerto), PRP (PRP = defeito + PRP) e autógeno + PRP (PRPA - defeito + enxerto + PRP). Todos os defeitos foram recobertos com uma barreira de PTFE e decorridos 15, 30 e 60 dias, 5 animais foram sacrificados por período, sendo as peças contendo os defeitos processadas para análises histológica e histomorfométrica. Outros 5 animais foram sacrificados aos 30 e 60 dias e submetidos à análise das propriedades biomecânicas e todos os espécimes foram submetidos ao exame radiográfico para análise da densidade óptica. Os resultados biomecânicos, radiográficos e histomorfométricos mostraram maior resistência, densidade óptica e maior formação óssea nos grupos A e PRPA quando comparados com os grupos C e PRP. Os resultados obtidos possibilitaram concluir que não houve uma melhora nos parâmetros radiográficos, mecânicos e na neoformação óssea quando o PRP foi usado isoladamente ou associado ao enxerto ósseo autógeno.
The aim of this study was to evaluate the effect of platelet rich plasma associated or not to autogenous bone graft in bone repair process of tibia rabbits surgical defects. In this research 25 adult rabbits were used, 2 defects were accomplished in each tibia, divided into groups: control (C = bone defect only filled out by blood coagulum), autogenous (A = bone defect + autogenous graft), PRP (PRP = bone defect + PRP) and autogenous + PRP (PRPA = bone defect + autogenous graft + PRP). All of the defects were covered with a PTFE barrier and after 15, 30 and 60 days there were elapsed. Five animals were sacrificed by period, being the pieces containing the defects processed for histological and histomorphometric analysis. Other 5 animals were sacrificed to the 30 and 60 days and submitted to biomechanics analysis and all of the specimens were submitted to the radiographic examination of the optical density. The biomechanic, radiographic and histomorphometric results showed larger resistance, optical density and better bone formation in the groups A and PRPA when compared with the groups C and PRP. This study demonstrated that there was not an improvement in the radiographic, mechanics, and bone formation parameters when PRP was used separately or associated to the autogenous bone graft.
The aim of this study was to evaluate the effect of platelet rich plasma associated or not to autogenous bone graft in bone repair process of tibia rabbits surgical defects. In this research 25 adult rabbits were used, 2 defects were accomplished in each tibia, divided into groups: control (C = bone defect only filled out by blood coagulum), autogenous (A = bone defect + autogenous graft), PRP (PRP = bone defect + PRP) and autogenous + PRP (PRPA = bone defect + autogenous graft + PRP). All of the defects were covered with a PTFE barrier and after 15, 30 and 60 days there were elapsed. Five animals were sacrificed by period, being the pieces containing the defects processed for histological and histomorphometric analysis. Other 5 animals were sacrificed to the 30 and 60 days and submitted to biomechanics analysis and all of the specimens were submitted to the radiographic examination of the optical density. The biomechanic, radiographic and histomorphometric results showed larger resistance, optical density and better bone formation in the groups A and PRPA when compared with the groups C and PRP. This study demonstrated that there was not an improvement in the radiographic, mechanics, and bone formation parameters when PRP was used separately or associated to the autogenous bone graft.
Descrição
Palavras-chave
Ossos - Enxerto, Ossos - Regeneração, Ossos - Cirurgia, Plasma rico em plaquetas, Platelet rich plasma, Bone graft, Bone regeneration
Como citar
MONTEIRO, Adriana do Socorro Ferreira. Efeito do plasma rico em plaquetas associado ao enxerto autógeno na tíbia de coelhos: estudos histomorfométrico e biomecânico. 2007. 122 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de São José dos Campos, 2007.