Proteínas do soro de queijo: hidrólise e formulação de suplemento alimentar para ratos Wistar
Data
2013-01-22
Autores
Barbosa, Ozeni Amorim [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O soro proveniente da fabricação de queijo tem sido utilizado para fins diversos, sendo uma importante fonte proteica de baixo custo apresentando alta percentagem de aminoácidos essenciais. Nesse contexto, a hidrólise de suas proteínas melhora a biodisponibilidade e permite que peptídeos bioativos formados sejam absorvidos. O objetivo desse estudo foi verificar a biodisponibilidade in vitro das proteínas do soro de queijo e seus hidrolisados com diferentes teores de hidrólise e o efeito da suplementação da dieta de ratos wistar adultos com esses hidrolisados. As soroproteínas foram submetidas a tratamento com as proteases pepsina, quimotripsina, carboxipeptidase-A e tripsina em pH e temperatura específicos, produzindo suplementos com médio (MTH) e alto (ATH) teor de hidrólise. A biodisponibilidade dos hidrolisados foi analisada por meio da simulação da digestão gástrica e duodenal. Os peptídeos e aminoácidos foram analisados por CLAE e o perfil proteico por SDS-PAGE. O perfil sérico, cálcio ósseo e a proteína muscular dos animais foram avaliados após suplementação com proteínas integrais e hidrolisados de alto e médio teor por um período de 43 dias. A biodisponibilidade de aminoácidos foi maior nos hidrolisados MTH e ATH após a simulação da digestão gastrointestinal, indicando que a hidrólise das soroproteínas pode aumentar a absorção destes compostos. O perfil sérico dos ratos apresentou alterações significativas principalmente em relação à glicemia após suplementação da dieta, sendo que os animais que receberam o suplemento MTH apresentaram uma grande redução nesse parâmetro e também excelente resultado em relação ao HDL, cálcio sérico e massa muscular. Embora de forma menos expressiva, os animais...
The whey from cheese production has been used for various purposes and it is an important low cost protein source with high proportion of essential amino acids. In this context, the hydrolysis of its proteins improves the bioavailability and allows the absorption of bioactive peptides formed. The aim of this research was to determine the in vitro bioavailability of whey proteins and their hydrolysates with different hydrolysis levels and the effect of supplementing the diet of adult Wistar rats with these hydrolysates. Whey proteins were subjected to treatment with proteases pepsin, trypsin, chymotrypsin, and carboxypeptidase A in specific pH and temperature, producing supplements with medium (MTH) and high (ATH) content of hydrolysis. The hydrolysates bioavailability was analyzed by simulating the gastric and duodenal digestion. Peptides and amino acids were analyzed by HPLC and protein profile by SDS-PAGE. The animals serum profile, bone calcium and muscle protein were evaluated after supplementation with integral proteins, high and medium hydrolyzed levels over a period of 43 days. The amino acid bioavailability was greater in hydrolysates MTH and ATH after simulating gastrointestinal digestion, indicating that the hydrolysis of whey proteins may increase the absorption of these compounds. The serum profile of the rats showed significant changes mainly related to glucose after dietary supplementation, and the animals that received the MTH supplement showed a large reduction in this parameter and also excellent results compared to HDL, serum calcium and muscle mass. The animals that received the ATH supplement showed satisfactory results for serum phosphorus and total protein. Thus, MTH supplement showed better results in terms of bioavailability and when used how supplement, and its excellent performance with respect... (Complete abstract click electronic access below)
The whey from cheese production has been used for various purposes and it is an important low cost protein source with high proportion of essential amino acids. In this context, the hydrolysis of its proteins improves the bioavailability and allows the absorption of bioactive peptides formed. The aim of this research was to determine the in vitro bioavailability of whey proteins and their hydrolysates with different hydrolysis levels and the effect of supplementing the diet of adult Wistar rats with these hydrolysates. Whey proteins were subjected to treatment with proteases pepsin, trypsin, chymotrypsin, and carboxypeptidase A in specific pH and temperature, producing supplements with medium (MTH) and high (ATH) content of hydrolysis. The hydrolysates bioavailability was analyzed by simulating the gastric and duodenal digestion. Peptides and amino acids were analyzed by HPLC and protein profile by SDS-PAGE. The animals serum profile, bone calcium and muscle protein were evaluated after supplementation with integral proteins, high and medium hydrolyzed levels over a period of 43 days. The amino acid bioavailability was greater in hydrolysates MTH and ATH after simulating gastrointestinal digestion, indicating that the hydrolysis of whey proteins may increase the absorption of these compounds. The serum profile of the rats showed significant changes mainly related to glucose after dietary supplementation, and the animals that received the MTH supplement showed a large reduction in this parameter and also excellent results compared to HDL, serum calcium and muscle mass. The animals that received the ATH supplement showed satisfactory results for serum phosphorus and total protein. Thus, MTH supplement showed better results in terms of bioavailability and when used how supplement, and its excellent performance with respect... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Peptídeos, Soro do leite, Suplementos dieteticos, Proteínas, Hidrolise, Hidrolisados de proteina, Cromatografia líquida de alta eficiência, Peptides
Como citar
BARBOSA, Ozeni Amorim. Proteínas do soro de queijo: hidrólise e formulação de suplemento alimentar para ratos Wistar. 2013. 92 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2013.