Influência da taurina na remodelação ventricular após o infarto do miocárdio
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Data
2012-12-12
Autores
Ardisson, Lidiane Paula [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do nosso estudo foi de avaliar a influência da administração de taurina sobre a remodelação após o infarto em ratos, por meio de análise de sobrevida e de variáveis morfológicas, funcionais, bioquímicas, celulares e intersticiais cardíacas. Foram utilizados ratos wistar, machos, entre 200 – 250g foram submetidos ao infarto experimental. Após 48hs do procedimento, os animais sobreviventes foram alocados em dois grupos aleatoriamente: grupo IAM (n=31), o qual recebeu água potável e grupo IAM-T (n=30), que recebeu 3% de taurina diluída na água. O grupo controle (n=10) foi composto por animais não infartados e recebeu água potável. Após 3 meses de acompanhamento foi realizado estudo morfológico e funcional pelos seguintes métodos: coração isolado, ecocardiograma, morfometria e histologia. O estudo bioquímico foi realizado por HPLC (para determinar as concentrações e taurina no plasma e no tecido cardíaco). A imunohistoquímica foi utilizada para avaliar a conexina 43 e apoptose. Por meio da zimografia avaliou-se as metaloproteases, por espectrofotometria foram avaliados estresse oxidativo e metabolismo energético e por western blot avaliou-se a resposta antioxidante. A análise estatística foi realizada pelo teste de ANOVA de uma via, t de Student, curva de Kaplan Méier e long – rank. O nível de significância adotado foi de 5%. A concentração de taurina plasmática (C = 49 (38 – 54,2) (μmol/L); IAM = 74,6 (58,7 – 83) (μmol/L); IAM-T = 363 (157 - 477,4) (μmol/L); p = 0,004) e no tecido cardíaco (C = 0,100 ± 0,04 (μmol/g); IAM = 0,175 ± 0,07 (μmol/g); IAM-T = 0,419± 0,187 (μmol/g); p = 0,022) foi maior no grupo IAM-T quando comparado com controle e IAM. Com relação ao estudo ecocardiográfico, o grupo IAM-T apresentou valores intermediários, quando comparado com os grupos C e IAM, nas variáveis AE (C = 5,3 ± 0,7 mm; IAM = 6,8 ± 1,0 mm; IAM-T = 6,8...
The aim of our study was to evaluate the influence of taurine administration on cardiac remodeling after myocardial infarction in rats by survival analysis and morphological, functional, biochemical, cellular and interstitial evalluation. Methods: Wistar male rats, weighting 200 - 250g were subjected to experimental myocardial infarction. 48 hours after the procedure, the surviving animals were randomly allocated into two groups: IAM group (n = 31), who received drinking tap water and T-IAM group (n = 30), who received 3% of taurine diluted in tap water. The control group (n = 10) was composed of non infarcted animals, who received drinking tap water. After 3 months of follow-up, morphological and functional study was conducted by the following. Isolated heart, echocardiography, histology and morphometry. Biochemical analysis was performed by HPLC (to determine the concentrations of taurine in plasma and heart tissue). Immunohistochemistry was used to evaluate connexin 43 and apoptosis. Metalloproteases was evaluated by zymography, oxidative stress and energy metabolism were evaluated by spectrophotometry and the antioxidant response, by western blot. Statistical analysis was performed by oneway ANOVA, Student t test, Kaplan Meier and long – rank tests. The level of significance was set at 5%. Concentration of taurine in plasma (C = 49 (38 - 54.2) (mmol / L), IAM = 74.6 (58.7 - 83) (mmol / L), T-IAM = 363 (157 - 477.4) (mmol / L), p = 0.004) and in heart tissue (C = 0.100 ± 0.04 (mmol / g), AMI = 0.175 ± 0.07 (mmol / g), T-IAM = 0.419 ± 0.187 (mol / g), p = 0.022) were higher in the T-IAM group compared with control and IAM groups. Regarding the echocardiographic study, the T-IAM group showed intermediate values when compared with C and IAM groups, variables LA (C = 5.3 ± 0.7 mm; IAM = 6.8 ± 1.0 mm; T-IAM = 6.1 ± 0.8 mm, p = <0.001), LA / tibia (C = 1.29 ± 0.18 mm / cm; IAM = 1.64 ± 0.25 mm / cm; ...
The aim of our study was to evaluate the influence of taurine administration on cardiac remodeling after myocardial infarction in rats by survival analysis and morphological, functional, biochemical, cellular and interstitial evalluation. Methods: Wistar male rats, weighting 200 - 250g were subjected to experimental myocardial infarction. 48 hours after the procedure, the surviving animals were randomly allocated into two groups: IAM group (n = 31), who received drinking tap water and T-IAM group (n = 30), who received 3% of taurine diluted in tap water. The control group (n = 10) was composed of non infarcted animals, who received drinking tap water. After 3 months of follow-up, morphological and functional study was conducted by the following. Isolated heart, echocardiography, histology and morphometry. Biochemical analysis was performed by HPLC (to determine the concentrations of taurine in plasma and heart tissue). Immunohistochemistry was used to evaluate connexin 43 and apoptosis. Metalloproteases was evaluated by zymography, oxidative stress and energy metabolism were evaluated by spectrophotometry and the antioxidant response, by western blot. Statistical analysis was performed by oneway ANOVA, Student t test, Kaplan Meier and long – rank tests. The level of significance was set at 5%. Concentration of taurine in plasma (C = 49 (38 - 54.2) (mmol / L), IAM = 74.6 (58.7 - 83) (mmol / L), T-IAM = 363 (157 - 477.4) (mmol / L), p = 0.004) and in heart tissue (C = 0.100 ± 0.04 (mmol / g), AMI = 0.175 ± 0.07 (mmol / g), T-IAM = 0.419 ± 0.187 (mol / g), p = 0.022) were higher in the T-IAM group compared with control and IAM groups. Regarding the echocardiographic study, the T-IAM group showed intermediate values when compared with C and IAM groups, variables LA (C = 5.3 ± 0.7 mm; IAM = 6.8 ± 1.0 mm; T-IAM = 6.1 ± 0.8 mm, p = <0.001), LA / tibia (C = 1.29 ± 0.18 mm / cm; IAM = 1.64 ± 0.25 mm / cm; ...
Descrição
Palavras-chave
Taurina - Efeito fisilógico, Coração - Ventrículo - Infarto, Apoptose, Stress oxidativo, Metabolismo energetico, Myocardial infarction
Como citar
ARDISSON, Lidiane Paula. Influência da taurina na remodelação ventricular após o infarto do miocárdio. 2012. 79 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, 2012.