Isolamentos e caracterização de DETs(Differentially Expressed Transcripts) em espécies de peixes: Leporinus macrocephalus(Characiformes) e Danio rerio (Cypriniformes)
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Data
2008-08-29
Autores
Costa, Fernanda Antunes Alves da [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O presente estudo teve o intuito de isolar e caracterizar transcritos diferencialmente expressos em espécies de peixes, visando contribuir com novos dados genéticos acerca deste grupo basal de vertebrados. Desta forma, diferentes análises foram realizadas em Leporinus macrocephalus (piauçu) (Characiformes, Anostomidae), espécie de peixe que representa um importante recurso alimentar que tem sido intensamente explorado na aqüicultura subtropical e que apresenta um sistema de determinação sexual ZZ/ZW, e também em Danio rerio (zebrafish) (Cypriniformes, Cyprinidae), um organismo modelo já bem estabelecido em diferentes áreas da pesquisa científica. Os resultados indicaram que: (1) As α-actinas esqueléticas de tecido muscular branco e vermelho parecem ser genética e funcionalmente distintas não somente em L. macrocephalus como também em outras espécies de peixes, característica que, até o momento, não foi encontrada em outro grupo de vertebrados. Além disso, as características de cada tipo de actina podem ser relacionadas às demandas energéticas dos tecidos musculares branco e vermelho; (2) Um expressão dimórfica entre machos e fêmeas ocorre em tecido cerebral de L. macrocephalus, relacionada ao gene que codifica a proteína APBA2 (amyloid beta (A4) precursor protein-binding, family A, member 2), que está associada à produção da proteína β-amilóide a qual, por sua vez, é relacionada à doença de Alzheimer; (3) A estrutura do gene fabp6 (fatty acid-binding protein 6) de D. rerio é similar à maioria dos outros membros da família multigênica fabp de outros vertebrados, e sua distribuição tecidual em embriões, larvas e adultos da espécie também é similar à encontrada em outros organismos, especialmente mamíferos; (4) Os genes que codificam a proteína p300 (E1A binding protein p300) e a proteína ADCY2 (adenilato ciclase 2) representam dois...
The present study intent to isolate and characterize differentially expressed transcripts in fish species in order to contribute to new genetic data on this basal vertebrate group. Therefore, different analyses were performed in Leporinus macrocephalus (piauçu) (Characiformes, Anostomidae), a fish species that represents an important food resource that has been intensively exploited in the subtropical aquaculture and that presents a ZZ/ZW sex determination system, and also in Danio rerio (zebrafish) (Cypriniformes, Cyprinidae), a well-established model organism in different areas of scientific research. The results indicated that: (1) The α-skeletal actins from white and red muscle tissues appear to be somewhat genetically and functionally distinguishable not only in L. macrocephalus but also in other fish species, a feature that was not found in other vertebrate groups so far. Moreover, the characteristics of each actin type could be related to the energetic demands of white and red muscle tissues; (2) A sexually dimorphic gene expression occurs in brain tissue of males and females of L. macrocephalus related to the gene that codes for the protein APBA2 (amyloid beta (A4) precursor protein-binding, family A, member 2), that is associated to the production of the β- amyloid protein which, in turns, is related to the Alzheimer disease; (3) The fabp6 (fatty acid-binding protein 6) gene structure of D. rerio is similar to most of the other members of the multigenic fabp family of other vertebrates, and its tissue distribution in embryos, larvae, and adults of the species is also similar to the one found in other organisms, specially mammals; (4) The genes that code for the protein p300 (E1A binding protein p300) and for the ADCY2 protein (adenylate cyclase 2) represent two candidate differentially expressed genes among nontransgenic and growth hormone (GH)-transgenic homozygote... (Complete abstract click electronic access below)
The present study intent to isolate and characterize differentially expressed transcripts in fish species in order to contribute to new genetic data on this basal vertebrate group. Therefore, different analyses were performed in Leporinus macrocephalus (piauçu) (Characiformes, Anostomidae), a fish species that represents an important food resource that has been intensively exploited in the subtropical aquaculture and that presents a ZZ/ZW sex determination system, and also in Danio rerio (zebrafish) (Cypriniformes, Cyprinidae), a well-established model organism in different areas of scientific research. The results indicated that: (1) The α-skeletal actins from white and red muscle tissues appear to be somewhat genetically and functionally distinguishable not only in L. macrocephalus but also in other fish species, a feature that was not found in other vertebrate groups so far. Moreover, the characteristics of each actin type could be related to the energetic demands of white and red muscle tissues; (2) A sexually dimorphic gene expression occurs in brain tissue of males and females of L. macrocephalus related to the gene that codes for the protein APBA2 (amyloid beta (A4) precursor protein-binding, family A, member 2), that is associated to the production of the β- amyloid protein which, in turns, is related to the Alzheimer disease; (3) The fabp6 (fatty acid-binding protein 6) gene structure of D. rerio is similar to most of the other members of the multigenic fabp family of other vertebrates, and its tissue distribution in embryos, larvae, and adults of the species is also similar to the one found in other organisms, specially mammals; (4) The genes that code for the protein p300 (E1A binding protein p300) and for the ADCY2 protein (adenylate cyclase 2) represent two candidate differentially expressed genes among nontransgenic and growth hormone (GH)-transgenic homozygote... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Genética, Peixes, Actina, Fish
Como citar
COSTA, Fernanda Antunes Alves da. Isolamentos e caracterização de DETs(Differentially Expressed Transcripts) em espécies de peixes: Leporinus macrocephalus(Characiformes) e Danio rerio (Cypriniformes). 2008. 172 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2008.