Resposta humoral de bovinos para os toxóides botulínicos C e D
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Data
2008-06-19
Autores
Curci, Vera Cláudia Lorenzetti Magalhães [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Foi avaliado pelo teste de ELISA indireto a resposta humoral para as toxinas botulínicas tipos C e D em animais vacinados com quatro diferentes produtos comerciais. Empregou-se duas vacinas bivalentes C e D (vacina 1 e vacina 2) e duas polivalentes contendo ainda os toxóides botulínicos tipos C e D (vacina 3 e vacina 4). Para análise foram realizadas seis colheitas de sangue ao longo do experimento, nos dias 0, 42, 75, 160, 250 e 342 após a vacinação. A imunidade passiva em bezerros até os 90 dias de idade, filhos de mães vacinadas com diferentes toxóides comerciais foram avaliados em 75 bezerros, agrupados de acordo com a vacina utilizada na mãe (B1, B2, B3 e B4). Para análise foram realizadas 3 colheitas de sangue, nos dias 5 (± 2), 45 e 90 dias após o nascimento. A avaliação da resposta humoral de bovinos vacinados em diferentes faixas etárias também foi realizada empregando-se uma vacina antibotulínica bivalente (C e D) comercial. Para análise, 90 bovinos foram divididos em 3 grupos de acordo com a faixa etária; animais com idade inferior a 2 anos, entre 2 e 5 anos e superior a 5 anos. Na avaliação da resposta humoral de animais vacinados com quatro diferentes produtos comerciais, para a toxina tipo C, as vacinas 1, 2, 3 e 4 não apresentaram diferenças significativas aos 42 dias da primeira dose. Aos 75 dias, a vacina 1, foi superior às vacinas 3 e 4, induzindo maior produção de anticorpos nos animais, porém não diferiu da vacina 2. Aos 160 dias houve queda na quantidade de anticorpos em todos os grupos, não havendo mais diferenças significativas entre as quatro vacinas testadas. Para a toxina tipo D, aos 42 dias da vacinação, não houve diferenças significativas entre as vacinas 1, 2 e 4, que apresentaram neste momento, valores superiores a vacina 3. No entanto, quando avaliadas aos 75 dias, a vacina 1 apresentou maior produção de anticorpos, diferindo...
To compare the efficiency of an “in house” ELISA test, standardized to measure antibody C and D from Clostridium botulinum, a survey to analysis four different commercial vaccines was conducted. The vaccines used were two commercial, bivalent botulinum containing subtypes C and D (vaccine 1 and vaccine 2) and other two polyvalent including subtypes C and D. The first trial was blood samples taken at 0, 42, 75, 160, 250 and 342 days after vaccination, whereas the booster was performed at day 42. The second trial, maternal antibodies were also measured taken blood samples from 1-3 months age steers obtained from vaccinated heifers with the same vaccines described above, and divided into four different groups (B1, B2, B3, B4). The blood samples were taken at days 5, 45 and 90 after birth. Third and last trial was humoral response from vaccinated cattle with different age evaluation performed using the commercial Clostridium botulinum vaccine subtype C and D. For this purpose, 90 animals never vaccinated, were divided into 3 groups: 1 - 2 years old; 2 – animals aged between 2 and 5 years old; 3 – animals 5 years old. The blood samples were taken 30 days after vaccination after second dose of the vaccine. From the first trial, no significant difference was observed when subtype C was search in sera from animals at 42 days after vaccination. However, at 75 days after vaccination, vaccine 1 was able to induce higher levels of antibodies when compared to vaccine 3 and 4. The antibody level was declining after 160 days post vaccination. For subtype D antigen, at 42 days after vaccination, no differences could be observed. However, at 75 day after vaccination, higher levels of antibodies were observed from animals vaccinated with vaccine 1. Comparing the bivalent vaccines, these were able to induce higher antibodies levels at 75 days after vaccination (toxoid C). In addition, the same... (Complete abstract click electronic access below)
To compare the efficiency of an “in house” ELISA test, standardized to measure antibody C and D from Clostridium botulinum, a survey to analysis four different commercial vaccines was conducted. The vaccines used were two commercial, bivalent botulinum containing subtypes C and D (vaccine 1 and vaccine 2) and other two polyvalent including subtypes C and D. The first trial was blood samples taken at 0, 42, 75, 160, 250 and 342 days after vaccination, whereas the booster was performed at day 42. The second trial, maternal antibodies were also measured taken blood samples from 1-3 months age steers obtained from vaccinated heifers with the same vaccines described above, and divided into four different groups (B1, B2, B3, B4). The blood samples were taken at days 5, 45 and 90 after birth. Third and last trial was humoral response from vaccinated cattle with different age evaluation performed using the commercial Clostridium botulinum vaccine subtype C and D. For this purpose, 90 animals never vaccinated, were divided into 3 groups: 1 - 2 years old; 2 – animals aged between 2 and 5 years old; 3 – animals 5 years old. The blood samples were taken 30 days after vaccination after second dose of the vaccine. From the first trial, no significant difference was observed when subtype C was search in sera from animals at 42 days after vaccination. However, at 75 days after vaccination, vaccine 1 was able to induce higher levels of antibodies when compared to vaccine 3 and 4. The antibody level was declining after 160 days post vaccination. For subtype D antigen, at 42 days after vaccination, no differences could be observed. However, at 75 day after vaccination, higher levels of antibodies were observed from animals vaccinated with vaccine 1. Comparing the bivalent vaccines, these were able to induce higher antibodies levels at 75 days after vaccination (toxoid C). In addition, the same... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Bovino, Botulismo, Vacinas, Teste imunoenzimático, Resposta humoral, Botulism, Bovine, Humoral response, Vaccine, ELISA
Como citar
CURCI, Vera Cláudia Lorenzetti Magalhães. Resposta humoral de bovinos para os toxóides botulínicos C e D. 2008. ix, 36 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2008.