Aderência in vitro de Streptococcus sanguinis e Candida albicans em implantes dentários de superfície lisa ou tratada
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Data
2009-07-14
Autores
Romeiro, Rogério de Lima [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo desse trabalho foi analisar in vitro a aderência de Streptococcus sanguinis, Candida albicans e associações destes microrganismos com Streptococcus mutans às superfícies dos implantes dentários tratados com jateamento de fosfato de cálcio, anodização, duplo ataque ácido e os de superfície lisa, com ou sem a prévia incubação em saliva ou plasma sanguíneo. Foram selecionados 120 implantes, sendo 30 de cada superfície para cada microrganismo e associações estudadas. Para análise da aderência, foram preparadas suspensões de microrganismos contendo 106 células/ml em espectrofotômetro. Além disso, cada microrganismo e associações foram divididos em três grupos: em um, o implante foi removido da embalagem e colocado diretamente no caldo, em outro, foi previamente embebido em saliva humana por uma hora e no último em plasma humano, também por uma hora. Os implantes foram acondicionados separadamente em poços de placas de cultura de células contendo caldo sacarosado (placa in vitro) e a suspensão do microrganismo. Após 24h de incubação a 37 °C e 5% de CO2, os implantes foram lavados três vezes durante um minuto em solução salina estéril e colocados em sonicador com 10 ml de salina para dispersão das células aderidas. A seguir, foram realizadas diluições seriadas e semeaduras em meios de cultura específico para cada microrganismo. Após 48h de incubação a 37 °C e 5% de CO2, foi realizada a contagem de unidades formadoras de colônias (UFC/ml) e os dados foram submetidos a análise de variância (ANOVA), teste de tukey, com nível de significância de 5%. Para ilustrar a aderência dos microrganismos, foram selecionados o microrganismo Streptococcus sanguinis, e as associações Streptococcus sanguinis e Candida albicans e Streptococcus sanguinis, Streptococcus mutans...
The aim of this study has been to analyze, in vitro the adherence of Streptococcus sanguinis, Candida albicans and associations of those microorganisms with Streptococcus mutans to the surfaces of dental implants treated with calcium phosphate jetting, anodization, double acid attack and to those of smooth surface, with or without previous saliva or blood plasma incubation. Ten implants from each surface were selected for every studied microorganism and association. In order to analyze the adherence, suspensions of microorganisms bearing 106 viable cells/ml in spectrophotometer were prepared. Additionally, every microorganism and association was divided in three groups: in the first, an implant was removed from its wrap and put right away into the sauce; the second, it was previously drenched into saliva for one hour; and the last one, into plasma, for one hour, as well. The implants were separately placed in culturing plaque wells of cells containing saccharose sauce (in vitro plaque) and the microorganism’s suspension. After 24 hours of incubation time at 37 °C and 5% of CO2, the implants were taken washed three times for a minute in saline sterile solution and put in a sonicator holding 10 ml of saline in order to disperse adherent cells. Then, seriated dilutions were done, and sowing in culture media specific for each of the. After a 48hincubation time at 37°C and 5% of CO2, a counting was carried, of the colony forming units (UFC/ml) and the data were submitted to the ANOVA, Tukey test, at a significance level of 5%. To illustrate the adherence of the microorganisms, some samples were exposed to electronic sweeping microscopy. The results did show great microorganism adherence to the surfaces studied, mainly when associated forming a biofilm. The anodized surface... (Complete abstract click electronic access below)
The aim of this study has been to analyze, in vitro the adherence of Streptococcus sanguinis, Candida albicans and associations of those microorganisms with Streptococcus mutans to the surfaces of dental implants treated with calcium phosphate jetting, anodization, double acid attack and to those of smooth surface, with or without previous saliva or blood plasma incubation. Ten implants from each surface were selected for every studied microorganism and association. In order to analyze the adherence, suspensions of microorganisms bearing 106 viable cells/ml in spectrophotometer were prepared. Additionally, every microorganism and association was divided in three groups: in the first, an implant was removed from its wrap and put right away into the sauce; the second, it was previously drenched into saliva for one hour; and the last one, into plasma, for one hour, as well. The implants were separately placed in culturing plaque wells of cells containing saccharose sauce (in vitro plaque) and the microorganism’s suspension. After 24 hours of incubation time at 37 °C and 5% of CO2, the implants were taken washed three times for a minute in saline sterile solution and put in a sonicator holding 10 ml of saline in order to disperse adherent cells. Then, seriated dilutions were done, and sowing in culture media specific for each of the. After a 48hincubation time at 37°C and 5% of CO2, a counting was carried, of the colony forming units (UFC/ml) and the data were submitted to the ANOVA, Tukey test, at a significance level of 5%. To illustrate the adherence of the microorganisms, some samples were exposed to electronic sweeping microscopy. The results did show great microorganism adherence to the surfaces studied, mainly when associated forming a biofilm. The anodized surface... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Implantes dentários, Streptococus mutans, Candida albicans, Streptococcus sanguinis, Implante dentário - Microorganismos, Dental implant - Microorganisms
Como citar
ROMEIRO, Rogério de Lima. Aderência in vitro de Streptococcus sanguinis e Candida albicans em implantes dentários de superfície lisa ou tratada. 2009. 97 f. Tese (doutorado) - Universidade Estadual Paulista,Faculdade de Odontologia de São José dos Campos., 2009.