Estudo do potencial de transformação maligna do líquen plano bucal: análise histológica, histoquímica e imunoistoquímica
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Data
2009-03-25
Autores
Sousa, Fernando Augusto Cervantes Garcia de [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo deste trabalho foi analisar possíveis alterações dos mecanismos de proliferação celular e apoptose no líquen plano bucal, comparando-as com as observadas na displasia epitelial e no carcinoma epidermóide, visando avaliar o seu potencial de transformação maligna. Foram selecionados 24 casos de cada lesão e a partir deles, novos cortes histológicos foram obtidos, sendo parte submetidos à técnica de AgNOR e parte à técnica imunoistoquímica da estreptoavidina-biotina-peroxidase para análise da expressão das proteínas PCNA, p53, bax e bcl-2. A média de NORs/núcleo no líquen plano bucal, na displasia epitelial e no carcinoma epidermóide bucal foram, respectivamente, 1,74±0,32, 2,42±0,62 e 2,41±0,61. A análise de variância revelou haver diferença estatisticamente significante entre o líquen plano bucal e as demais lesões (p<0,05). Quanto à PCNA, p53, bax e bcl-2, o teste de qui-quadrado não revelou haver diferença significante entre a expressão da p53 e da bcl-2. Contudo, a expressão da PCNA foi significativamente menor no líquen plano bucal do que nas demais lesões. Não houve diferença estaticamente significante entre a expressão da PCNA e da bax na displasia epitelial e no carcinoma epidermóide bucal. Conclui-se que alterações na expressão destas proteínas podem ser observadas tanto no líquen plano bucal quanto na displasia epitelial e no carcinoma epidermóide bucal, sugerindo o potencial de transformação maligna desta lesão e enfatizando a importância do acompanhamento em longo prazo dos pacientes. Neste contexto, a análise das NORs se mostrou ferramenta útil em avaliar os casos de líquen plano bucal que apresentem maior risco de transformação maligna.
The purpose of this study was to analyze possible alterations in the mechanisms of cell proliferation and apoptosis in oral lichen planus, comparing them with those observed in epithelial dysplasia and squamous cell carcinoma, aiming to establish its malignant transformation potential. Twenty-four cases of each lesion were selected and new histological cuts were obtained, being part of the cuts submitted to the AgNOR and part to the immunohistochemical technique of streptoavidine-biotin technique for analysis of the expression of the PCNA, bax and bcl-2 proteins. The NORs/nucleous mean for oral lichen planus, epithelial dysplasia and squamous cell carcinoma was respectively 1.74±0.32, 2.42±0.62 and 2.41±0.61. Analysis of variance showed statistically significant difference between the oral lichen planus and the others lesions (p<0.05). Regarding PCNA, p53, bax and bcl-2, chi-squared test showed no significant differences between the expression of p53 and bcl-2. However, the expression of PCNA was significantly lower in oral lichen planus than in epithelial dysplasia and oral squamous cell carcinoma. No significant differences between the expression of PCNA and bax in epithelial dysplasia and oral squamous cell carcinoma were observed. It was concluded that alterations in the expression of these proteins can be observed in oral lichen planus, suggesting the potential of malignant transformation of this lesion and emphasizing the importance of follow-up of patients. Within this context, the NORs analysis is a useful tool to evaluate the oral lichen planus cases with high risk of malignant transformation.
The purpose of this study was to analyze possible alterations in the mechanisms of cell proliferation and apoptosis in oral lichen planus, comparing them with those observed in epithelial dysplasia and squamous cell carcinoma, aiming to establish its malignant transformation potential. Twenty-four cases of each lesion were selected and new histological cuts were obtained, being part of the cuts submitted to the AgNOR and part to the immunohistochemical technique of streptoavidine-biotin technique for analysis of the expression of the PCNA, bax and bcl-2 proteins. The NORs/nucleous mean for oral lichen planus, epithelial dysplasia and squamous cell carcinoma was respectively 1.74±0.32, 2.42±0.62 and 2.41±0.61. Analysis of variance showed statistically significant difference between the oral lichen planus and the others lesions (p<0.05). Regarding PCNA, p53, bax and bcl-2, chi-squared test showed no significant differences between the expression of p53 and bcl-2. However, the expression of PCNA was significantly lower in oral lichen planus than in epithelial dysplasia and oral squamous cell carcinoma. No significant differences between the expression of PCNA and bax in epithelial dysplasia and oral squamous cell carcinoma were observed. It was concluded that alterations in the expression of these proteins can be observed in oral lichen planus, suggesting the potential of malignant transformation of this lesion and emphasizing the importance of follow-up of patients. Within this context, the NORs analysis is a useful tool to evaluate the oral lichen planus cases with high risk of malignant transformation.
Descrição
Palavras-chave
Mucosa bucal, Carcinoma de celulas escamosas, Carcinoma epidermóide, Oral lichen planus
Como citar
SOUSA, Fernando Augusto Cervantes Garcia de. Estudo do potencial de transformação maligna do líquen plano bucal: análise histológica, histoquímica e imunoistoquímica. 2009. 79 f. Tese (doutorado) – Universidade Estadual Paulista, Faculdade de Odontologia de São José dos Campos, 2009.