Estudo comparativo da resposta inflamatória crônica induzida em Piaractus mesopotamicus e Oreochromis niloticus por corpo estranho e bacilo de Calmette-Guérin
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Data
2012-12-14
Autores
Gómez Manrique, Wilson [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A piscicultura está caracterizada pela alta densidade de estocagem, transporte e altos níveis de arraçoamento, alterando a qualidade de água e a homeostase dos peixes. Uma das principais características frente a estas alterações é a resposta geral de adaptação, aumentando a susceptibilidade dos peixes às doenças infecciosas e parasitárias ao mesmo tempo em que a má qualidade da água particularmente quando rica em matéria orgânica, favorece a proliferação de agentes com potencial. Um dos processos fisiopatológicos de grande importância para manter a saúde do hospedeiro é a resposta inflamatória aguda que se cronifica com a persistência do agente. O implante de lamínulas de vidro no tecido subcutâneo de peixes induz a resposta inflamatória crônica, apresentando acúmulo de macrófagos e formação de células gigantes multinucleadas. O inóculo do BCG induz intenso infiltrado mononuclear com desorganização do tecido causado por edema com dissociação das fibras musculares e necrose. O surgimento de células gigantes ocorre de forma progressiva e com o tempo estas aumentam de tamanho e número de núcleos formando células gigantes tipo corpo estranho. Com o avanço do processo a disposição dos macrófagos ao redor do inóculo é maior e surgem fibroblastos, linfócitos e na maioria dos casos presença de melanomacrófagos formando o granuloma. Neste trabalho objetivou-se caracterizar morfologicamente o processo inflamatório crônico por corpo estranho induzido pelo implante de lamínulas de vidro no tecido subcutâneo e o epitelióide induzido pelo inóculo de BCG em Piaractus mesopotamicus e Oreochromis niloticus. 180 peixes de cada gênero foram utilizados no estudo, divididos em quatro grupos de 45 cada. Os tratamentos foram: Inoculados, Implantados, Inoculado e Implantados e grupo controle. Nos grupos...
Fish farming is characterized by high stocking density, transport and high levels of feeding, changing water quality and fish homeostasis. A major characteristic of these changes is opposite the overall response adjustment, increasing the susceptibility of fish to infectious and parasitic diseases at the same time that poor water quality particularly when rich in organic matter, favors the proliferation of agents with potential. One of the pathophysiological processes of major importance for maintaining the health of the host is the acute inflammatory response that chronic the persistence of the agent. The implantation of glass coverslips in the subcutaneous tissue of fish induces chronic inflammatory response, with accumulation of macrophages and multinucleated giant cell formation. The inoculum of BCG induces intense mononuclear infiltrate with disruption caused by tissue dissociation with edema and necrosis of muscle fibers. The emergence of giant cells occurs gradually and over time they increase in size and number of cores forming giant cells of foreign body type. With the progress of the process layout of macrophages around the inoculum is larger and appear fibroblasts, lymphocytes and in most cases the presence of melanomacrophages forming the granuloma. This study aimed to characterize morphologically the chronic inflammatory process induced by foreign body implant glass slides in the subcutaneous tissue and epithelioid induced by inoculation of BCG in Piaractus mesopotamicus and Oreochromis niloticus. 180 fish in each sex were used in the study were divided into four groups of 45 each. The treatments were: inoculated, deployed, deployed and Inoculated and control group). In inoculated groups were given 20 μl of BCG vaccine (40 mg/mL) (n° bacilli than 2.0 x 106 CFU / mg of BCG strain Mureau Rio de Janeiro) in skeletal striated... (Complete abstract click electronic access below)
Fish farming is characterized by high stocking density, transport and high levels of feeding, changing water quality and fish homeostasis. A major characteristic of these changes is opposite the overall response adjustment, increasing the susceptibility of fish to infectious and parasitic diseases at the same time that poor water quality particularly when rich in organic matter, favors the proliferation of agents with potential. One of the pathophysiological processes of major importance for maintaining the health of the host is the acute inflammatory response that chronic the persistence of the agent. The implantation of glass coverslips in the subcutaneous tissue of fish induces chronic inflammatory response, with accumulation of macrophages and multinucleated giant cell formation. The inoculum of BCG induces intense mononuclear infiltrate with disruption caused by tissue dissociation with edema and necrosis of muscle fibers. The emergence of giant cells occurs gradually and over time they increase in size and number of cores forming giant cells of foreign body type. With the progress of the process layout of macrophages around the inoculum is larger and appear fibroblasts, lymphocytes and in most cases the presence of melanomacrophages forming the granuloma. This study aimed to characterize morphologically the chronic inflammatory process induced by foreign body implant glass slides in the subcutaneous tissue and epithelioid induced by inoculation of BCG in Piaractus mesopotamicus and Oreochromis niloticus. 180 fish in each sex were used in the study were divided into four groups of 45 each. The treatments were: inoculated, deployed, deployed and Inoculated and control group). In inoculated groups were given 20 μl of BCG vaccine (40 mg/mL) (n° bacilli than 2.0 x 106 CFU / mg of BCG strain Mureau Rio de Janeiro) in skeletal striated... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Peixe, Pacu (Peixe), Granuloma, Imuno-histoquímica, Teleósteo, BCG, Fishes
Como citar
GÓMEZ MANRIQUE, Wilson. Estudo comparativo da resposta inflamatória crônica induzida em Piaractus mesopotamicus e Oreochromis niloticus por corpo estranho e bacilo de Calmette-Guérin. 2012. xxii, 69 p. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2012.