Identificação de marcas moleculares ligadas à ausência de sementes em videira
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Data
2002-04-11
Autores
Silva, Ana Veruska Cruz da [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A videira é uma frutífera de grande importância mundial e, atualmente, a ausência de sementes tem sido uma característica bastante exigida pelos consumidores de uvas de mesa. O objetivo do presente trabalho foi a identificação de marcas moleculares associadas à ausência de sementes, utilizando as técnicas RAPD e fAFLP. O material vegetal foi oriundo dos bancos de germoplasma da EMBRAPA (CPATSA e CNPUV), totalizando 19 cultivares, umas com sementes, outras sem sementes e ainda, três porta-enxertos. Para a análise RAPD foram utilizados 213, onde apenas 30 possibilitaram amplificação de todas as amostras, sendo estes os usados para a obtenção dos dendrogramas. Esses primers produziram um total de 337 bandas polimórficas, tendo sido possível encontrar uma marca específica para a ausência de sementes, utilizando o primer UBC 443, que poderá futuramente ser utilizada para o desenvolvimento de marcadores SCAR, possibilitando a criação de um kit de identificação rápida e precoce de apirenia em videira. A análise fAFLP proporcionou a visualização de um dendrograma onde verificou-se que as cultivares foram agrupadas especificamente, ou seja, separados em cultivares com sementes, sem sementes e os porta enxertos. Foi possível identificar uma marca molecular específica para a característica de ausência de sementes, utilizando o marcador RAPD e, com o fAFLP, obteve-se um melhor agrupamento das cultivares estudadas.
The grapevine is one of the most important fruit world-wide and, actually, the absence of seeds has been a desirable feature by table grapes consumers. This research had as an objective, the identification of molecular marks associated to the absence of seeds, using RAPD and fAFLP techniques. The vegetal material used were proceeding from the germoplasm banks of EMBRAPA (CPATSA and CNPUV). It was used 19 cultivars, with and without seeds, and three different rootstocks. For RAPD analysis it was used 30 primers, producing a total of 337 polymorphics bands, it was also possible to find a specific mark for the absence of seeds, using primer UBC 443, that it could be used in the future for the development of SCAR markers, making possible the creation of a kit of fast and precocious identification of absence of seeds in grapevine. The fAFLP analysis provided a visualization of a dendrogram where it was verified that the cultivars were arranged in specific groups, separated in three different cultivars: with seeds, without seeds and the rootstocks. It was possible to identify a specific molecular mark for the feature of absence of seeds, using RAPD marker and, with fAFLP, to obtain a better arrangement of these cultivars.
The grapevine is one of the most important fruit world-wide and, actually, the absence of seeds has been a desirable feature by table grapes consumers. This research had as an objective, the identification of molecular marks associated to the absence of seeds, using RAPD and fAFLP techniques. The vegetal material used were proceeding from the germoplasm banks of EMBRAPA (CPATSA and CNPUV). It was used 19 cultivars, with and without seeds, and three different rootstocks. For RAPD analysis it was used 30 primers, producing a total of 337 polymorphics bands, it was also possible to find a specific mark for the absence of seeds, using primer UBC 443, that it could be used in the future for the development of SCAR markers, making possible the creation of a kit of fast and precocious identification of absence of seeds in grapevine. The fAFLP analysis provided a visualization of a dendrogram where it was verified that the cultivars were arranged in specific groups, separated in three different cultivars: with seeds, without seeds and the rootstocks. It was possible to identify a specific molecular mark for the feature of absence of seeds, using RAPD marker and, with fAFLP, to obtain a better arrangement of these cultivars.
Descrição
Palavras-chave
Uva, Porta-enxertos, Sementes, Seed
Como citar
SILVA, Ana Veruska Cruz da. Identificação de marcas moleculares ligadas à ausência de sementes em videira. 2002. iv, 58 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2002.