Efeito da implantação iônica por imersão em plasmas sobre a bioatividade de titânio
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Data
2012-07-31
Autores
Rangel, Rita de Cássia Cipriano [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Neste trabalho a técnica de Implantação Iônica e Deposição por Imersão em Plasma, IIDIP, foi utilizada para deposição de filmes contendo cálcio sobre a superfície de titânio. Os parâmetros de deposição foram variados buscando-se condições que tornassem o titânio o mais bioativo possível. Para a deposição dos filmes contendo cálcio foram utilizados, como precursores, vapor de nitrato de cálcio dissolvido em álcool isopropílico ou cálcio granulado. Para avaliar a bioatividade dos filmes as amostras foram imersas em solução corpórea simulada, SBF. Espectrometria de emissão de raios X induzida por partículas carregadas (PIXE), espectroscopia de reflexão/absorção no infravermelho(IRRAS), espectroscopia de fotoelétrons de raios-x (XPS), difração de raios X (DRX) e espectroscopia de energia dispersiva (EDS) foram utilizadas para análises da composição química e estrutura molecular dos filmes depositados. A morfologia da superfície foi avaliada por microscopia eletrônica de varredura (MEV) e microscopia de força atômica (AFM). A espessura dos filmes foi medida por perfilometria, enquanto a energia de superfície e ângulo de contato foram obtidos pelo método da gota séssil em um goniômetro automatizado. Células osteoblásticas foram semeadas sobre amostras de titânio recobertas com o filme a plasma e controle e cultivadas para investigação da adesão e viabilidade celulares. Espectroscopia de impedância eletroquímica foi utilizada para avaliar modificações algumas amostras de titânio após a deposição a plasma e a imersão em SBF. Os resultados mostraram melhora na bioatividade do material, sendo que a viabilidade de células osteoblásticas aumentou mais de 100% para uma amostra de titânio tratada por IIDIP a partir de vapor da solução de nitrato de cálcio. Esse aumento na viabilidade foi atribuído a elevados... (Resumo completo, clicar acesso eletrônico
In this work Plasma Immersion Ion Implantation and Deposition, PIID, technique was employed for deposition of calcium-containing films on titanium surfaces. The deposition parameters have been varied in search of conditions that would enhance the bioactivity of titanium surfaces as much as possible. Vapor of solution of calcium nitrate dissolved in isopropyl alcohol, or the sublimation of granulated metalic calcium were used as Ca precursors. The superficial modifications were investigated before and after soaking the samples in SBF. Particle-induced x-ray emission (PIXE), infrared reflectance/absorbance (IRRAS), x-ray photoelectron (XPS), x-ray difraction (XRD) and energy-dispersive x-ray (EDS) spectroscopies were used to characterize the chemical composition and molecular structure of the films. The surface morphology was evaluated by scanning electron microscopy (MEV) and atomic force microscopy (AFM). The thickness of the films was measurement by profilometry, while the surface energy and contact angle were assessed using the sessible drop method in an automated goniometer. Osteoblast cells was seeded on the coated titanium and controls and cultured to investigate cell adhesion and viability. Electrochemical Impedance Spectroscopy, EIS, was used to evaluate changes after plasma deposition and soaking in SBF. Osteoblast cells viability increased 100% in a titanium sample treated by PIIID from vapor of calcium nitrate solution. The increasing in cell viability has been ascribed to high values of roughness and surface energy. Calcium and phosphorous were detected in samples treated by PIIID from granulated calcium after soaking in SBF. Micrographs of those surfaces have revealed morphologies typical of apatite, which presence has been confirmed by XPS and XRD. High resolution XPS spectra... (Complete abstract click electronic access below)
In this work Plasma Immersion Ion Implantation and Deposition, PIID, technique was employed for deposition of calcium-containing films on titanium surfaces. The deposition parameters have been varied in search of conditions that would enhance the bioactivity of titanium surfaces as much as possible. Vapor of solution of calcium nitrate dissolved in isopropyl alcohol, or the sublimation of granulated metalic calcium were used as Ca precursors. The superficial modifications were investigated before and after soaking the samples in SBF. Particle-induced x-ray emission (PIXE), infrared reflectance/absorbance (IRRAS), x-ray photoelectron (XPS), x-ray difraction (XRD) and energy-dispersive x-ray (EDS) spectroscopies were used to characterize the chemical composition and molecular structure of the films. The surface morphology was evaluated by scanning electron microscopy (MEV) and atomic force microscopy (AFM). The thickness of the films was measurement by profilometry, while the surface energy and contact angle were assessed using the sessible drop method in an automated goniometer. Osteoblast cells was seeded on the coated titanium and controls and cultured to investigate cell adhesion and viability. Electrochemical Impedance Spectroscopy, EIS, was used to evaluate changes after plasma deposition and soaking in SBF. Osteoblast cells viability increased 100% in a titanium sample treated by PIIID from vapor of calcium nitrate solution. The increasing in cell viability has been ascribed to high values of roughness and surface energy. Calcium and phosphorous were detected in samples treated by PIIID from granulated calcium after soaking in SBF. Micrographs of those surfaces have revealed morphologies typical of apatite, which presence has been confirmed by XPS and XRD. High resolution XPS spectra... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Titanio, Calcio, Implantação iônica, Deposição química de vapor, Espectroscopia de infravermelho, Raios X - Difração, Titanium, Calcium, Ion implantation, Chemical vapor deposition, Infrared spectroscopy, X-rays - Diffraction
Como citar
RANGEL, Rita de Cássia Cipriano. Efeito da implantação iônica por imersão em plasmas sobre a bioatividade de titânio. 2012. 100 f. Tese (doutorado) - Universidade Estadual Paulista, Unidade Diferenciada de Sorocaba/Iperó, 2012.