Determinação das doses letal 50 e 100 do propofol em nanoemulsão ou em emulsão lipídica pela via intraperitoneal em camundongos
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Data
2012-01-01
Autores
Gehrcke, Martielo Ivan
da Rosa, Ademir Cassiano [UNESP]
Tamanho, Renato Batista
de Moraes, Aury Nunes
Oleskovicz, Nilson
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual de Londrina (UEL)
Resumo
The formulation of a drug can interfere with its absorption into the circulatory system and may result in changes in the dose required to achieve that particular effect. The aim of this study was to determine the lethal dose 50 (LD 50) and 100 (LD100) of a nanoemulsion of propofol and the lipid emulsion in mice intraperitoneally. One hundred sixty animals weighing 36.47 +/- 4.6g, which were distributed randomly into two groups: NANO and EMU who received propofol 1% in the nanoemulsion and lipid emulsion, respectively, intraperitoneally. Began with a dose of 250mg/kg (n=10) and from this isdecreased or increased the dose until achieving 0 and 100% of deaths in each group thus formed were seven subgroups in NANO (each subgroup n = 10) at doses 200, 250, 325, 350, 400, 425 and 475 mg/kg and in EMU eight subgroups (n= 10 each subset) 250, 325, 350, 400, 425, 475, 525 and 575 mg/kg. In the CONTROL group (n= 10) animals received saline in the largest volume used in the other groups to rule out death by the volume injected. Analysis of LD 50 and LD 100 were obtained by linear regression. The LD 50 was 320, 95 mg / kg and 4243, 51mg / kg and the LD 100 was445.99 mg / kg and 595.31 mg / kg to groups NANO and EMU, respectively. It follows that nanoemulsion is propofol in 25% more potent compared to the lipid emulsionintraperitoneally.
Descrição
Palavras-chave
Lethal dose, potency, propofol, nanoemulsion, mice
Como citar
Semina-ciencias Agrarias. Londrina: Universidade Estadual de Londrina (UEL), v. 33, n. 5, p. 1911-1917, 2012.