Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
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Data
2010-01-21
Autores
Ferrasi, Adriana Camargo [UNESP]
Pinheiro, Nidia Alice [UNESP]
Barem Rabenhorst, Silvia Helena
Caballero, Otavia Luisa
Rodrigues, Maria Aparecida Marchesan [UNESP]
de Carvalho, Fabricio
Leite, Celso Vieira de Souza [UNESP]
Pitombeira Ferreira, Marcia Valeria
Pessoa Barros, Marcos Aurelio
Pardini, Maria Ines de Moura Campos [UNESP]
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Título de Volume
Editor
W J G Press
Resumo
AIM: To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA(+) and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H, pylori and genotyping were performed by PCR, using specific primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the chi(2) or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA(+) in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H, pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. (C) 2010 Baishideng. All rights reserved.
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Palavras-chave
Gastric cancer, Methylation, Microsatellite instability, Helicobacter pylori, Epstein Barr virus
Como citar
World Journal of Gastroenterology. Beijing: W J G Press, v. 16, n. 3, p. 312-319, 2010.