Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices
Nenhuma Miniatura disponível
Data
2014-07-01
Autores
Pessan, Juliano Pelim [UNESP]
Ribeiro Pinto Alves, Karina Mirela [UNESP]
Italiani, Flavia de Moraes
Ramires, Irene
Pereira Lauris, Jose Roberto
Whitford, Gary Milton
Toumba, Kyriacos Jack
Robinson, Colin
Rabelo Buzalaf, Marilia Afonso
Título da Revista
ISSN da Revista
Título de Volume
Editor
Wiley-Blackwell
Resumo
Background. The distribution of fluoride and calcium in plaque after the use of fluoride dentifrices has not yet been determined.Aim. To evaluate fluoride and calcium distribution in sections of biofilms generated in situ after the use of conventional and low-fluoride dentifrices.Design. Children (n = 11, 8-10 years old) brushed with placebo (fluoride-free), low-fluoride (513 mgF/kg), and conventional (1072 mgF/kg) dentifrices twice daily for 1 week, following a double-blind, cross-over protocol. Biofilms were generated using Leeds in situ devices, which were collected 1 and 12 h after brushing, and sectioned through their depth. Sections were grouped (10 x 5 mu m) for fluoride and calcium analysis. Sections 4 mu m thick were used for image analysis and determination of biomass fraction. Results were analysed by ANOVA, Tukey's test, and linear regression analysis (P < 0.05).Results. Fluoride and calcium were mostly located at the outer sections of biofilms for all dentifrices tested, and these ions were directly correlated throughout most of biofilm's sections. Results for conventional dentifrice were significantly higher than for the placebo, but did not differ from those for the low-fluoride dentifrice.Conclusions. The use of a low-fluoride dentifrice did not promote a higher fluoride uptake in inner biofilms' sections, as hypothesized. As plaque fluoride was significantly elevated only after the use of the conventional dentifrice, the recommendation of low-fluoride formulations should be done with caution, considering both risks and benefits.
Descrição
Palavras-chave
Como citar
International Journal Of Paediatric Dentistry. Hoboken: Wiley-blackwell, v. 24, n. 4, p. 293-302, 2014.